Division of Physical Science and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Chakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bang Phli, Samut Prakarn, Thailand.
Nat Prod Res. 2022 Nov;36(21):5462-5469. doi: 10.1080/14786419.2021.2015593. Epub 2021 Dec 13.
Two new compounds, one -pyrone (trichoharzianone) and one decalin (trichoharzianin), along with eight known compounds including three decalins, two -lactones, two carboxylic acids and one isochroman were isolated from the marine-derived fungus PSU-MF79. The structures were determined by spectroscopic methods. The relative configuration of trichoharzianin was assigned based on NOEDIFF data and coupling constants whereas the absolute configurations were established by comparison of electronic circular dichroism data with those of the co-metabolites. Known (-)-massoia lactone exhibited mild antifungal activity against ATCC90113 flucytosine-resistant, ATCC90028 and NCPF3153 with MIC values of 128, 200 and 200 µg/mL, respectively, and weak cytotoxic activity against HCT-116 and MCF-7 cell lines with the respective IC values of 17 and 32 µM. In addition, it was noncytotoxic against noncancerous Vero cells with an IC value of >100 µM.
从海洋来源真菌 PSU-MF79 中分离得到了两种新化合物，一种 - 吡喃酮（曲哈那酮）和一种十氢化萘（曲哈那嗪），以及包括三种十氢化萘、两种内酯、两种羧酸和一种异色满在内的八种已知化合物。通过光谱方法确定了结构。基于 NOEDIFF 数据和偶合常数确定了曲哈那嗪的相对构型，而绝对构型则通过与共代谢物的电子圆二色性数据进行比较来确定。已知的（-）- 马索内酯对 ATCC90113 氟胞嘧啶耐药、ATCC90028 和 NCPF3153 表现出温和的抗真菌活性，MIC 值分别为 128、200 和 200 µg/mL，对 HCT-116 和 MCF-7 细胞系的细胞毒性活性较弱，IC 值分别为 17 和 32 µM。此外，它对非癌细胞 Vero 无细胞毒性，IC 值大于 100 µM。
