Kuhr W G, Wightman R M
Brain Res. 1986 Aug 27;381(1):168-71. doi: 10.1016/0006-8993(86)90707-9.
Dopamine release at the submicromolar level has been observed in the striatum of an anesthetized rat on a millisecond time scale. Fast-scan cyclic voltammetry with Nafion-coated microelectrodes has been synchronized with electrical stimulation of the medial forebrain bundle, and synaptic overflow is observed following a burst of 15 impulses. The rapid appearance of dopamine following this stimulus indicates that the source of dopamine is very close (approximately 10 micron) to the electrode. The rapid disappearance of released dopamine reflects the potency of cellular uptake for dopamine. Inhibition of dopamine uptake with nomifensine allows the measurement of dopamine overflow as a result of a single stimulus impulse or with low-frequency stimulations, both comparable to physiological dopaminergic impulse flow.
在麻醉大鼠的纹状体中，已观察到亚微摩尔水平的多巴胺在毫秒时间尺度上释放。用涂有Nafion的微电极进行的快速扫描循环伏安法已与内侧前脑束的电刺激同步，并且在15次脉冲爆发后观察到突触溢出。该刺激后多巴胺的快速出现表明多巴胺的来源非常靠近（约10微米）电极。释放的多巴胺的快速消失反映了细胞对多巴胺摄取的能力。用诺米芬辛抑制多巴胺摄取可测量由于单个刺激脉冲或低频刺激导致的多巴胺溢出，这两者都与生理性多巴胺能冲动流相当。
