Ali A, Ali R
Clin Biochem. 1986 Aug;19(4):205-8. doi: 10.1016/s0009-9120(86)80026-1.
A rapid, simple and highly sensitive enzyme-linked immunosorbent assay (ELISA) utilizing nylon as the solid support is described for the detection of anti-DNA antibodies in autoimmune disorders. The optimal reaction conditions were established with an anti-DNA antibody-positive SLE serum. Fifty-three percent of systemic lupus erythematosus patients, 29 percent of patients with systemic lupus erythematosus with overlapping progressive systemic sclerosis and 10 percent of progressive systemic sclerosis patients were positive for anti-DNA antibodies. The sensitivity of the method was compared with passive hemagglutination and fluorometric assays, the latter using ethidium bromide as intercalating dye. The method described is specific, reproducible and convenient for use in clinical laboratories where large numbers of samples are to be screened for the detection of anti-DNA antibodies. The procedure requires small quantities of antigen or antibody and is, therefore, highly economical.
本文描述了一种以尼龙为固相载体的快速、简单且高度灵敏的酶联免疫吸附测定法（ELISA），用于检测自身免疫性疾病中的抗DNA抗体。采用抗DNA抗体阳性的系统性红斑狼疮（SLE）血清确定了最佳反应条件。53%的系统性红斑狼疮患者、29%的系统性红斑狼疮合并进行性系统性硬化症患者以及10%的进行性系统性硬化症患者抗DNA抗体呈阳性。将该方法的灵敏度与被动血凝试验和荧光测定法进行了比较，后者使用溴化乙锭作为嵌入染料。所描述的方法具有特异性、可重复性，便于在临床实验室中用于筛查大量样本以检测抗DNA抗体。该方法所需的抗原或抗体量少，因此非常经济。
