Montecucco C, Bestagno M, Cerino A, Caporali R, Carnevale R, Longhi M, Pedrini M A, Astaldi-Ricotti G C
Dipartimento di Medicina Interna e Terapia Medica, Universita' di Pavia, Italy.
Clin Exp Rheumatol. 1989 Jan-Feb;7(1):5-11.
A quantitative immunoassay using a highly purified antigen from HeLa cells has allowed us to detect antibodies to SSB/La in 11/20 patients with primary Sjögren's syndrome (SS), 15/33 with SLE, and 11/35 with progressive systemic sclerosis (PSS). However, positive results were found in only 2/12 patients with idiopathic Raynaud's disease and 2/20 with rheumatoid arthritis, including 4 with secondary SS. Anti-SSB/La were associated with extraglandular manifestations in primary SS, and with a diffuse sclerodermic pattern in PSS. In SS, SLE and PSS, a positive anti-SSB/La test was strongly associated with nodal or spleen enlargement and with an increased level of gamma-globulins. A direct association was also found with positive tests for rheumatoid factors, anti-SSA/Ro and anti-Scl 70. An inverse relationship was however found with anti-nRNP +/- Sm and anticentromere antibodies. Our data suggest that anti-SSB/La antibodies can be regarded as a marker of B-lymphocyte activation in patients with either primary SS, SLE or PSS.
使用来自人宫颈癌细胞系（HeLa细胞）的高度纯化抗原来进行定量免疫测定，使我们能够在20例原发性干燥综合征（SS）患者中的11例、33例系统性红斑狼疮（SLE）患者中的15例以及35例进行性系统性硬化症（PSS）患者中的11例检测到抗SSB/La抗体。然而，仅在12例特发性雷诺病患者中的2例以及20例类风湿关节炎患者（包括4例继发性SS患者）中的2例发现阳性结果。抗SSB/La与原发性SS中的腺体外表现相关，与PSS中的弥漫性硬皮病模式相关。在SS、SLE和PSS中，抗SSB/La检测呈阳性与淋巴结或脾脏肿大以及γ球蛋白水平升高密切相关。还发现与类风湿因子、抗SSA/Ro和抗Scl 70检测呈阳性直接相关。然而，与抗nRNP+/-Sm和抗着丝点抗体呈负相关。我们的数据表明，抗SSB/La抗体可被视为原发性SS、SLE或PSS患者B淋巴细胞活化的标志物。
