以尼龙为固相载体，采用酶联免疫吸附测定法检测系统性红斑狼疮患者血清中的抗RNA抗体。
Detection of anti-RNA antibodies in systemic lupus erythematosus by ELISA using nylon as solid support.
作者信息
Kumar A, Ali R
出版信息
Immunol Lett. 1984;7(5):293-6. doi: 10.1016/0165-2478(84)90038-5.
PMID:6202626
Abstract
A highly sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the detection of anti-RNA antibodies is described. The assay procedure involves adsorption of total cellular RNA on nylon beads which could be conveniently stored for a considerable period of time without loss in antigenicity. Sixty-four percent of systemic lupus erythematosus (SLE) sera were positive for anti-RNA antibodies with fluorogenic substrate against 48% with colorigenic substrate.
摘要
本文描述了一种用于检测抗RNA抗体的高灵敏度且可重复的酶联免疫吸附测定（ELISA）方法。该测定方法包括将总细胞RNA吸附在尼龙珠上，这些尼龙珠可以方便地储存相当长一段时间而不会丧失抗原性。64%的系统性红斑狼疮（SLE）血清抗RNA抗体检测呈阳性（使用荧光底物），而使用显色底物时这一比例为48%。
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