Selka M A, Chenafa A, Achouri M Y
Pharmacognosy laboratory, pharmacy department, faculty of medicine, TOXIMED laboratory, university of Tlemcen, 123 Hamri Ahmed road, Tlemcen, Algérie.
Galenic pharmacy laboratory, pharmacy department, faculty of medicine, university of Sidi Bel Abbes, Colonel Othmane avenue, Sidi Bel Abbes, Algérie.
Ann Pharm Fr. 2022 Jul;80(4):507-518. doi: 10.1016/j.pharma.2021.11.006. Epub 2021 Dec 9.
Clove essential oil is isolated from the dried flower buds of Syzygium aromaticum from which many biological activities have been reported. The aim of this work was to develop a cream for topical use containing clove essential oil (HE) as an active ingredient and to test its biological activity.
Clove EO was extracted by hydrodistillation, its chemical composition was analysed by Gas chromatography-mass spectrometry (CG-SM). The EO was then incorporated into a cream where its stability was studied. The antifungal activity was evaluated by disc-diffusion method. The in vivo anti-inflammatory activity of EO and the cream was assessed y by carrageenan-induced rat paw edema model.
Clove EO contains two major constituents: eugenol (69.14%) and β-Caryophyllene (18.8%). The cream had good organoleptic and physicochemical properties. Both EO and the cream showed growth inhibitory effects on the two studied dermatophytes strains. The incorporation of EO in the cream provided a good anti-inflammatory activity, similar to the reference drug.
The developed creme was stable, had good anti-inflammatory properties and moderate antifungal activity. Long-term stability studies and in vivo tests would lead to the use of clove essential oil in the development of new pharmaceutical forms.
丁香精油是从丁香的干燥花蕾中提取的，已有许多关于其生物活性的报道。本研究的目的是开发一种含有丁香精油（HE）作为活性成分的外用乳膏，并测试其生物活性。
采用水蒸馏法提取丁香精油，通过气相色谱-质谱联用（CG-SM）分析其化学成分。然后将该精油加入乳膏中并研究其稳定性。采用纸片扩散法评估抗真菌活性。通过角叉菜胶诱导的大鼠足趾肿胀模型评估精油及乳膏的体内抗炎活性。
丁香精油含有两种主要成分：丁香酚（69.14%）和β-石竹烯（18.8%）。该乳膏具有良好的感官和理化性质。精油和乳膏对两种受试皮肤癣菌菌株均显示出生长抑制作用。将精油加入乳膏中具有良好的抗炎活性，与参比药物相似。
所开发的乳膏稳定，具有良好的抗炎性能和适度的抗真菌活性。长期稳定性研究和体内试验将有助于丁香精油在新型药物剂型开发中的应用。
