Achraf Abdou, Fatima Ezzahra Maaghloud, Fatima Zahra Moukhfi, Abdoullah Bimoussa, Nour-Eddine Chadli, Abdelhakim Elmakssoudi, Jamal Jamal Eddine, Mohamed Dakir
Laboratory of Organic Synthesis, Extraction and Valorization, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Morocco.
Laboratory of Immunology & Biodiversity Faculty of Sciences Aïn Chock, Hassan II University of Casablanca, Morocco.
Chem Biodivers. 2023 Apr;20(4):e202201034. doi: 10.1002/cbdv.202201034. Epub 2023 Mar 17.
Clove bud is a medicinal plant used traditionally in Asia for the treatment of various disease. Previously, Clove oil is a potential source of an antimicrobial compounds especially vis-a-vis bacterial pathogens. However, the compound responsible for this activity remains to be investigated. Essential oil (EO) clove, acetylated essential oil clove, eugenol, and acetyleugenol were evaluate as an antibacterial potential agent against Staphyloccocus aureus (SE), Escherichia coli (EC) and Pseudomonas aeruginosa (PA). Essential oil containing eugenol was extracted from buds of Eugenia caryophyllata commonly named clove (Syzygium aromaticum (L.) (Family Myrtaceae) by a simple hydrodistillation. The analysis of the essential oils (EOs) using gas chromatography-mass spectrometry (GC-MS) shows eugenol as the major constituent with 70.14 % of the total. The Eugenol was isolated from the EO using chemical treatment. Afterwards, the EO and eugenol were converted to acetylated EO and acetyleugenol, respectively using acetic anhydride. The antibacterial result revealed that all compounds showed a strong activity against the three strains. The Staphyloccocus aureus and Pseudomonas aeruginosa were extremely sensitive against eugenol with an inhibition diameters of 25 mm. The MIC values of eugenol versus S. aureus and P. aeruginosa were 0.58 and 2.32 mg/mL, respectively, while the MIB values were 2.32 mg/mL and 9.28 mg/mL.
丁香花蕾是一种传统上在亚洲用于治疗各种疾病的药用植物。以前，丁香油是抗菌化合物的潜在来源，特别是对细菌病原体而言。然而，负责这种活性的化合物仍有待研究。对丁香精油（EO）、乙酰化丁香精油、丁香酚和乙酰丁香酚作为针对金黄色葡萄球菌（SE）、大肠杆菌（EC）和铜绿假单胞菌（PA）的抗菌潜在剂进行了评估。从通常称为丁香（丁香属（L.）（桃金娘科））的丁香 Eugenia caryophyllata 的花蕾中通过简单的水蒸馏提取含丁香酚的精油。使用气相色谱 - 质谱联用（GC - MS）对精油（EOs）进行分析表明，丁香酚是主要成分，占总量的70.14％。使用化学处理从EO中分离出丁香酚。之后，分别使用乙酸酐将EO和丁香酚转化为乙酰化EO和乙酰丁香酚。抗菌结果表明，所有化合物对这三种菌株均表现出强活性。金黄色葡萄球菌和铜绿假单胞菌对丁香酚极其敏感，抑菌直径为25毫米。丁香酚对金黄色葡萄球菌和铜绿假单胞菌的MIC值分别为0.58和2.32毫克/毫升，而MIB值分别为2.32毫克/毫升和9.28毫克/毫升。
