Schilham M W, Lang R, Benner R, Zinkernagel R M, Hengartner H
J Immunol. 1986 Nov 1;137(9):2748-54.
An alloreactive cytotoxic T cell clone (433) possessing the L3T4-, Lyt-2+ phenotype is described that shows a double specificity. It has been derived from unprimed BALB/c (H-2d) spleen cells by repetitive in vitro restimulation with C57BL/6(H-2b) cells. The specificity of clone 433 was determined in cytotoxicity and proliferation experiments. One specificity was for the class I antigen H-2Db and the second was for the class II antigen I-Ek. Inhibition of cytotoxicity with monoclonal antibodies confirmed these results. Cold target competition experiments demonstrated that the two specificities were mediated by the same cell population. Anti-Lyt-2 antibodies inhibited only the H-2Db- but not the I-Ek-specific lysis, suggesting a higher affinity of the antigen receptor for I-Ek than for Db. To our knowledge, this is the first description of a T cell clone that is specific for a class I antigen and cross-reacts heteroclitically with a class II antigen.
描述了一种具有L3T4-、Lyt-2+表型的同种异体反应性细胞毒性T细胞克隆（433），该克隆表现出双重特异性。它是通过用C57BL/6（H-2b）细胞进行重复体外再刺激，从未致敏的BALB/c（H-2d）脾细胞中获得的。在细胞毒性和增殖实验中确定了克隆433的特异性。一种特异性针对I类抗原H-2Db，第二种特异性针对II类抗原I-Ek。用单克隆抗体抑制细胞毒性证实了这些结果。冷靶竞争实验表明，这两种特异性是由同一细胞群体介导的。抗Lyt-2抗体仅抑制H-2Db特异性裂解，而不抑制I-Ek特异性裂解，这表明抗原受体对I-Ek的亲和力高于对Db的亲和力。据我们所知，这是对一种对I类抗原具有特异性并与II类抗原发生交叉反应的T细胞克隆的首次描述。
