Perkins D L, Lai M Z, Smith J A, Gefter M L
Department of Biology Massachusetts Institute of Technology, Cambridge 02139.
J Exp Med. 1989 Jul 1;170(1):279-89. doi: 10.1084/jem.170.1.279.
Previous data from many groups show that both class I and class II-restricted T cells recognize short synthetic peptides in the context of their respective MHC molecules (9-18), all of the peptides described to date are restricted to only a single class of MHC molecules; however, structural homology between the class I and II MHC molecules and the use of similar TCRs by class I and II-restricted T cells suggest that antigen recognition mechanisms are similar in both systems. To directly compare antigen recognition in the two systems, we analyzed peptides for the ability to function in both a class I and II-restricted system and found that seven of seven individual peptides tested stimulate both class I and II-restricted T cell responses. In addition, two of the peptides can function in different species stimulating both human class I and murine class II T cell responses. Thus, the process of T cell recognition of antigen in the context of MHC molecules was highly conserved in evolution not only between the class I and class II MHC systems, but also between the murine and human species.
许多研究小组之前的数据表明，I类和II类限制性T细胞在各自的MHC分子背景下识别短合成肽（9 - 18），迄今为止所描述的所有肽都仅局限于单一类别的MHC分子；然而，I类和II类MHC分子之间的结构同源性以及I类和II类限制性T细胞对相似TCR的使用表明，两个系统中的抗原识别机制相似。为了直接比较两个系统中的抗原识别，我们分析了肽在I类和II类限制性系统中发挥作用的能力，发现所测试的七个单独肽中有七个能刺激I类和II类限制性T细胞反应。此外，其中两个肽可以在不同物种中发挥作用，刺激人类I类和小鼠II类T细胞反应。因此，在MHC分子背景下T细胞识别抗原的过程在进化中高度保守，不仅在I类和II类MHC系统之间，而且在小鼠和人类物种之间也是如此。
