Harrington James M, Haines Laura G, Essader Amal S, Liyanapatirana Chamindu, Poitras Eric A, Weber Frank X, Levine Keith E, Fernando Reshan A, Robinson Veronica G, Waidyanatha Suramya
Analytical Sciences, RTI International, Research Triangle Park, NC, USA.
Division of the National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA.
Anal Lett. 2021;54(17):2777-2788. doi: 10.1080/00032719.2021.1890107. Epub 2021 Mar 1.
Human exposure to vanadium (V) is anticipated because it is a drinking water contaminant. Due to limited data on soluble V salts, the National Toxicology Program is investigating the toxicity in rodents following drinking water exposure. Measurement of internal V dose allows for interpretation of toxicology data. The objective of this study was to develop and validate an inductively coupled plasma-mass spectrometric method to quantitate total V in rat plasma. The method was linear (r ≥ 0.99) from 5.00 - 1,000 ng V/mL. Intra- and inter-day relative error (% RE) and relative standard deviation (% RSD) of spiked plasma samples were 8.5% - 15.6% RE and ≤ 1.8% RSD and 7.3% - 11.7% RE and ≤ 3.1% RSD, respectively. The limit of detection was 0.268 ng V/mL plasma and absolute percent recovery was 113%. Standards up to 7,500 ng V/mL plasma were diluted into the validated range (5.6% RE, 0.9% RSD). V in extracted plasma samples over 15 days at ambient and refrigerated conditions was from 97.7 - 126% of day 0. Determined plasma V concentrations after three freeze-thaw cycles and after frozen storage for up to 63 days ranged from 100 - 106% and 100 - 122% of day 0, respectively. The method was extended to rat urine (accuracy and precision -2.0 - 0.3% RE and <0.6% RSD, respectively for same linear range). These data demonstrate that the method is suitable to quantitate V in rat plasma and urine.
由于钒（V）是一种饮用水污染物，因此预计人类会接触到它。由于可溶性钒盐的数据有限，国家毒理学计划正在研究饮用水暴露后啮齿动物的毒性。测量体内钒剂量有助于解释毒理学数据。本研究的目的是开发并验证一种电感耦合等离子体质谱法，用于定量大鼠血浆中的总钒。该方法在5.00 - 1,000 ng V/mL范围内呈线性（r≥0.99）。加标血浆样品的日内和日间相对误差（% RE）和相对标准偏差（% RSD）分别为8.5% - 15.6% RE和≤1.8% RSD以及7.3% - 11.7% RE和≤3.1% RSD。检测限为0.268 ng V/mL血浆，绝对回收率为113%。高达7,500 ng V/mL血浆的标准品被稀释至验证范围内（相对误差5.6%，相对标准偏差0.9%）。在环境温度和冷藏条件下，提取的血浆样品在15天内的钒含量为第0天的97.7 - 126%。经过三个冻融循环和长达63天的冷冻保存后，测定的血浆钒浓度分别为第0天的100 - 106%和100 - 122%。该方法扩展至大鼠尿液（在相同线性范围内，准确度和精密度分别为-2.0 - 0.3% RE和<0.6% RSD）。这些数据表明该方法适用于定量大鼠血浆和尿液中的钒。
