Lübeck Institute for Experimental Dermatology (LIED), University of Lübeck, Lübeck, Germany.
Center for Blistering Diseases, University of Groningen, Groningen, Netherlands.
Front Immunol. 2021 Nov 25;12:773720. doi: 10.3389/fimmu.2021.773720. eCollection 2021.
Anti-laminin 332 mucous membrane pemphigoid (MMP) is an autoimmune blistering disease characterized by predominant mucosal lesions and autoantibodies against laminin 332. The exact diagnosis of anti-laminin 332 MMP is important since nearly 30% of patients develop solid cancers. This study compared two independently developed diagnostic indirect immunofluorescence (IF) tests based on recombinant laminin 332 expressed in HEK239 cells (biochip mosaic assay) and the migration trails of cultured keratinocytes rich in laminin 332 (footprint assay). The sera of 54 anti-laminin 332 MMP, 35 non-anti-laminin 332 MMP, and 30 pemphigus vulgaris patients as well as 20 healthy blood donors were analyzed blindly and independently. Fifty-two of 54 and 54/54 anti-laminin 332 MMP sera were positive in the biochip mosaic and the footprint assay, respectively. In the 35 non-anti-laminin 332 MMP sera, 3 were positive in both tests and 4 others showed weak reactivity in the footprint assay. In conclusion, both assays are easy to perform, highly sensitive, and specific, which will further facilitate the diagnosis of anti-laminin 332 MMP.
抗层粘连蛋白 332 黏膜性大疱性皮病（MMP）是一种自身免疫性水疱病，以主要黏膜损伤和抗层粘连蛋白 332 自身抗体为特征。抗层粘连蛋白 332 MMP 的明确诊断很重要，因为近 30%的患者会发展为实体瘤。本研究比较了两种独立开发的基于重组层粘连蛋白 332 的诊断性间接免疫荧光（IF）检测，该蛋白在 HEK239 细胞中表达（生物芯片镶嵌试验）和富含层粘连蛋白 332 的培养角质形成细胞的迁移轨迹（足迹试验）。对 54 例抗层粘连蛋白 332 MMP、35 例非抗层粘连蛋白 332 MMP 和 30 例寻常性天疱疮患者以及 20 例健康献血者的血清进行了盲法和独立分析。生物芯片镶嵌试验中，54 例抗层粘连蛋白 332 MMP 血清中的 52 例和 54/54 例为阳性，足迹试验中，54 例抗层粘连蛋白 332 MMP 血清中的 52 例和 54/54 例为阳性。在 35 例非抗层粘连蛋白 332 MMP 血清中，两种检测均有 3 例阳性，另外 4 例在足迹试验中表现出弱阳性反应。总之，这两种检测方法都易于操作、高度敏感且特异性高，这将进一步促进抗层粘连蛋白 332 MMP 的诊断。
