Sarkar Chiranjib, Parsad Rajender, Mishra Dwijesh C, Rai Anil
ICAR-Indian Agricultural Research Institute, New Delhi, India.
ICAR-Indian Agricultural Statistics Research Institute, New Delhi, India.
Front Genet. 2021 Nov 24;12:745827. doi: 10.3389/fgene.2021.745827. eCollection 2021.
Gene regulatory network (GRN) construction involves various steps of complex computational steps. This step-by-step procedure requires prior knowledge of programming languages such as R. Development of a web tool may reduce this complexity in the analysis steps which can be easy accessible for the user. In this study, a web tool for constructing consensus GRN by combining the outcomes obtained from four methods, namely, correlation, principal component regression, partial least square, and ridge regression, has been developed. We have designed the web tool with an interactive and user-friendly web page using the php programming language. We have used R script for the analysis steps which run in the background of the user interface. Users can upload gene expression data for constructing consensus GRN. The output obtained from analysis will be available in downloadable form in the result window of the web tool.
基因调控网络（GRN）构建涉及众多复杂的计算步骤。这种循序渐进的过程需要诸如R等编程语言的先验知识。开发一个网络工具可能会降低分析步骤中的这种复杂性，用户可以轻松访问该工具。在本研究中，已开发出一种通过结合四种方法（即相关性、主成分回归、偏最小二乘法和岭回归）所得结果来构建共识GRN的网络工具。我们使用php编程语言设计了一个具有交互式且用户友好的网页的网络工具。我们在用户界面的后台运行的分析步骤中使用了R脚本。用户可以上传基因表达数据来构建共识GRN。分析获得的输出将以可下载的形式在网络工具的结果窗口中提供。
