Kusmardi Kusmardi, Azzahra Baihaqi Lyanna, Estuningtyas Ari, Sahar Nurhuda, Sunaryo Hadi, Tedjo Aryo
Department of Anatomic Pathology, Faculty of Medicine, Universitas Indonesia, Jl. Salemba Raya 6, Jakarta, Indonesia.
Drug Development Research Center (DDRC Cluster, IMERI, Faculty of Medicine), Jakarta, Indonesia.
Int J Inflam. 2021 Dec 2;2021:4919410. doi: 10.1155/2021/4919410. eCollection 2021.
Colorectal cancer (CRC) is a malignancy derived from the glandular epithelial cells in the colon. Patients with inflammatory bowel disease (IBD) are more likely to develop CRC. Cancer proliferation is characterized by the loss of inhibition of apoptosis, which involves caspase-3 activation. This study examined the effects of the pomegranate peel extract on the expression of caspase-3 in mice crypt cells induced by dextran sodium sulfate (DSS) 2%.
The experimental study was done in six groups. All treatments were done in 42 days. The groups were all induced by DSS through water drinking, except for the normal group, which was only given water. The treatments given included the pomegranate extract in two doses (240 mg and 480 mg/kg bw/day), aspirin, and ellagic acid. The specimens were then fixated and stained for the immunohistochemistry scoring for the expression of caspase-3, which was then analyzed statistically.
The H-scores of each treatment group were 213.23 ± 8.32 (DSS group), 243.81 ± 18.69 (normal group), 226.10 ± 12.38 (pomegranate peel extract of 240 mg/kg/d), 238.84 ± 15.81 (pomegranate peel extract of 480 mg/kg/d), 227.47 ± 12.15 (aspirin), and 224.01 ± 18.39 (ellagic acid). Statistical differences were found in one-way analysis of variance (ANOVA) and analysis among the DSS group, normal group, and dose 2 group (pomegranate peel extract of 480 mg/kg/day).
The ethanol extract of pomegranate was able to induce apoptosis, which was demonstrated by the increase of caspase-3 expression.
结直肠癌（CRC）是一种起源于结肠腺上皮细胞的恶性肿瘤。炎症性肠病（IBD）患者更易患结直肠癌。癌症增殖的特征是凋亡抑制的丧失，这涉及半胱天冬酶-3的激活。本研究检测了石榴皮提取物对2%葡聚糖硫酸钠（DSS）诱导的小鼠隐窝细胞中半胱天冬酶-3表达的影响。
实验研究分为六组。所有处理均持续42天。除正常组仅给予水外，其余各组均通过饮水给予DSS诱导。给予的处理包括两种剂量的石榴提取物（240mg和480mg/kg体重/天）、阿司匹林和鞣花酸。然后将标本固定并染色，进行免疫组织化学评分以检测半胱天冬酶-3的表达，随后进行统计学分析。
各处理组的H评分分别为213.23±8.32（DSS组）、243.81±18.69（正常组）、226.10±12.38（240mg/kg/d石榴皮提取物组）、238.84±15.81（480mg/kg/d石榴皮提取物组）、227.47±12.15（阿司匹林组）和224.01±18.39（鞣花酸组）。在单向方差分析（ANOVA）以及DSS组、正常组和剂量2组（480mg/kg/天石榴皮提取物组）之间的分析中发现了统计学差异。
石榴乙醇提取物能够诱导凋亡，这通过半胱天冬酶-3表达的增加得到证实。
