Miró Guadalupe, Regidor-Cerrillo Javier, Checa Rocio, Diezma-Díaz Carlos, Montoya Ana, García-Cantalejo Jesús, Botías Pedro, Arroyo Javier, Ortega-Mora Luis-Miguel
Pet Parasite Lab, Department of Animal Health, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.
Saluvet-Innova S.L., Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.
Front Vet Sci. 2021 Nov 10;8:779341. doi: 10.3389/fvets.2021.779341. eCollection 2021.
In this study, we describe SARS-CoV-2 infection dynamics in one cat and three dogs from households with confirmed human cases of COVID-19 living in the Madrid Community (Spain) at the time of expansion (December 2020 through June 2021) of the alpha variant (lineage B.1.1.7). A thorough physical exam and nasopharyngeal, oropharyngeal, and rectal swabs were collected for real-time reverse-transcription PCR (RT-qPCR) SARS-CoV-2 testing on day 0 and in successive samplings on days 7, 14, 21, and 47 during monitoring. Blood was also drawn to determine complete blood counts, biochemical profiles, and serology of the IgG response against SARS-CoV-2. On day 0, the cat case 1 presented with dyspnea and fever associated with a mild bronchoalveolar pattern. The dog cases 2, 3, and 4 were healthy, but case 2 presented with coughing, dyspnea, and weakness, and case 4 exhibited coughing and bilateral nasal discharge 3 and 6 days before the clinical exam. Case 3 (from the same household as case 2) remained asymptomatic. SARS-CoV-2 detection by RT-qPCR showed that the cat case 1 and the dog case 2 exhibited the lowest cycle threshold (Ct) (Ct < 30) when they presented clinical signs. Viral detection failed in successive samplings. Serological analyses revealed a positive IgG response in cat case 1 and dog cases 3 and 4 shortly after or simultaneously to virus shedding. Dog case 2 was seronegative, but seroconverted 21 days after SARS-CoV-2 detection. SARS-CoV-2 genome sequencing was attempted, and genomes were classified as belonging to the B.1.1.7 lineage.
在本研究中，我们描述了在阿尔法变异株（谱系B.1.1.7）传播期间（2020年12月至2021年6月），来自西班牙马德里自治区有确诊COVID-19病例家庭的1只猫和3只狗的SARS-CoV-2感染动态。在监测的第0天以及随后第7、14、21和47天进行连续采样时，采集了全面的体格检查样本以及鼻咽、口咽和直肠拭子，用于SARS-CoV-2的实时逆转录PCR（RT-qPCR）检测。还采集了血液以确定全血细胞计数、生化指标以及针对SARS-CoV-2的IgG反应血清学。第0天，猫病例1出现呼吸困难和发热，伴有轻度支气管肺泡病变。狗病例2、3和4健康，但病例2在临床检查前3天出现咳嗽、呼吸困难和虚弱，病例4在临床检查前3天和6天出现咳嗽和双侧鼻分泌物。病例3（与病例2来自同一家庭）无症状。RT-qPCR检测SARS-CoV-2显示，猫病例1和狗病例2出现临床症状时循环阈值（Ct）最低（Ct < 30）。后续采样未检测到病毒。血清学分析显示，猫病例1以及狗病例3和4在病毒脱落不久后或同时出现IgG反应阳性。狗病例2血清学阴性，但在检测到SARS-CoV-2后21天血清转化。尝试进行了SARS-CoV-2基因组测序，基因组被分类为属于B.1.1.7谱系。
