Kumar Sushant, Madhav N V Satheesh
Faculty of Pharmacy, Pharmacy College Saifai, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, U.P., 26130, India.
Faculty of Pharmacy, DIT Univesity, Dehradun, U.K., India.
Iran J Pharm Res. 2021 Summer;20(3):78-93. doi: 10.22037/ijpr.2021.113734.14457.
The research aims to develop bionanosuspension using a biopolymer isolated from fruit that constitutes potential and natural polymeric properties. At first, the biomaterial was isolated from the natural fruit pulp of by an economical method of isolation. The model drug was nanosized by a novel sonication method. The isolated biopolymer was characterized for its polymeric properties, and its potential capabilities were evaluated in the delivery of nanosized phenytoin. The isolated biopolymer was characterized for DSC, FTIR, NMR, mass, and scanning electron microscopy. The isolated biomaterial was used for the preparation of phenytoin-loaded bionanosuspension with other excipients. The bionanoparticles were also characterized by different analytical testing such as FTIR, DSC, and SEM to confirm any interaction between model drug and biopolymer. The prepared bionanoparticles showed the release of phenytoin in a sustained manner over 36 hours. The release kinetic study was done using the BIT-SOFT 1.12 software and other parameters such as t50%, t80%, and were calculated. The formulation PFr6 was considered best having t50% in 18.22 hours and t80% in 29.62 h with an r value of 0.9793. This formulation showed up to 87.89% drug release within 36 hours. The prepared bio-nanosuspension was found to be stable and in a well-dispersed state. The dried bionanosuspension evaluation revealed no interaction between model drug and biopolymer without any loss of characteristic peaks. Therefore, the isolated biopolymer can be safely used to prepare stable bionanosuspension loaded with nanosized phenytoin.
该研究旨在利用从水果中分离出的具有潜在天然聚合物特性的生物聚合物开发生物纳米混悬液。首先，通过一种经济的分离方法从天然果肉中分离出生物材料。采用新型超声处理方法将模型药物纳米化。对分离出的生物聚合物的聚合特性进行了表征，并评估了其在纳米尺寸苯妥英钠递送中的潜在能力。对分离出的生物聚合物进行了差示扫描量热法（DSC）、傅里叶变换红外光谱法（FTIR）、核磁共振（NMR）、质谱和扫描电子显微镜表征。将分离出的生物材料与其他辅料用于制备载苯妥英钠的生物纳米混悬液。还通过FTIR、DSC和SEM等不同分析测试对生物纳米颗粒进行了表征，以确认模型药物与生物聚合物之间的任何相互作用。制备的生物纳米颗粒在36小时内显示出苯妥英钠的持续释放。使用BIT - SOFT 1.12软件进行释放动力学研究，并计算t50%、t80%等其他参数。制剂PFr6被认为是最佳制剂，其t50%为18.22小时，t80%为29.62小时，r值为0.9793。该制剂在36小时内显示出高达87.89%的药物释放。发现制备的生物纳米混悬液稳定且处于良好分散状态。干燥的生物纳米混悬液评估显示模型药物与生物聚合物之间没有相互作用，且特征峰没有任何损失。因此，分离出的生物聚合物可安全用于制备载纳米尺寸苯妥英钠的稳定生物纳米混悬液。
