Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, 25240, Erzurum, Turkey.
High Technology Research and Application Centre, Erzurum Technical University, Erzurum, Turkey.
Curr Microbiol. 2021 Dec 14;79(1):15. doi: 10.1007/s00284-021-02694-4.
Infections from multi-drug resistant bacteria and biofilms constitute a serious problem worldwide. There is a need for new antibacterial and antibiofilm compounds in the fight against infectious diseases. In recent years, pigment-producing microorganisms have drawn a great deal of attention as a promising source for antibacterial and antibiofilm compounds. Here, we report the antibacterial and antibiofilm activity of pigments synthesized by bacteria isolated from soil. This study aimed to perform an evaluation of the antibacterial, antibiofilm, and characteristic of crude pigments from Rhodococcus sp. SC1 isolates. The total pigment extract exhibited antibacterial activity against Gram-positive and Gram-negative reference bacteria with required minimum inhibitory concentration (MIC) values ranging from 64 to 256 µg/ml. Moreover, it reduced biofilm formation of Gram-negative reference bacteria at sub-MIC concentration. For characterization of the pigments, UV-absorbance, thin layer chromatography, fourier transform infrared spectroscopy, and QTOF-LC/MS analyses were performed. The results of this study showed that pigments of Rhodococcus sp. SC1 isolates can be a candidate for medical applications.
多药耐药菌和生物膜感染是一个全球性的严重问题。在与传染病作斗争时，需要新的抗菌和抗生物膜化合物。近年来，产色素微生物作为一种有前途的抗菌和抗生物膜化合物的来源，引起了极大的关注。在这里，我们报告了从土壤中分离的细菌合成的色素的抗菌和抗生物膜活性。本研究旨在评估 Rhodococcus sp. SC1 分离株合成的粗色素的抗菌、抗生物膜特性。总色素提取物对革兰氏阳性和革兰氏阴性参考菌表现出抗菌活性，所需最低抑菌浓度 (MIC) 值范围为 64 至 256μg/ml。此外，它在亚 MIC 浓度下减少了革兰氏阴性参考菌的生物膜形成。为了对色素进行表征，进行了紫外吸收、薄层层析、傅里叶变换红外光谱和 QTOF-LC/MS 分析。这项研究的结果表明，Rhodococcus sp. SC1 分离株的色素可以成为医疗应用的候选物。
