Alrowaili Majed G, Hussein Abdelaziz M, Eid Elsayed A, Serria Mohamed S, Abdellatif Hussein, Sakr Hussein F
Department of Surgery (Orthopedic Division), Faculty of Medicine, Northern Border University, Arar, Saudi Arabia.
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Bone Metab. 2021 Nov;28(4):307-316. doi: 10.11005/jbm.2021.28.4.307. Epub 2021 Nov 30.
The present study examined the effect of intermittent fasting (IF) on bone mineral content (BMC) and bone mineral density (BMD) and the markers of bone remodeling in a glucocorticoid-induced osteoporosis (GIO) rat model.
Forty male rats were allocated to 4 groups (N=10 per group): control group of normal rats; control+IF group (normal rats subjected to IF for 16-18 hr daily for 90 days); dexamethasone (DEX) group: (DEX [0.5 mg i.p.] for 90 days); and DEX+IF group (DEX and IF for 90 days). By the end of the experiment, BMD and BMC in the right tibia were measured. Serum levels of the following were measured: glucose; insulin; triglycerides (TGs); total cholesterol; parathyroid hormone (PTH); osteoprotegerin (OPG); receptor activator of nuclear factor-κB (RANK); bone-resorbing cytokines, including bone deoxypyridinoline (DPD), N-terminal telopeptide of collagen type I (NTX-1), and tartrate-resistant acid phosphatase 5b (TRAP-5b); and bone-forming cytokines, including alkaline phosphatase (ALP) and osteocalcin (OC).
DEX administration for 90 days resulted in significantly increased serum levels of glucose, insulin, TGs, cholesterol, PTH, OPG, DPD, NTX-1, and TRAP-5b and significantly decreased BMD, BMC, and serum levels of RANK, OC, and ALP (all P<0.05). IF for 90 days significantly improved all these parameters (all P<0.05).
IF corrected GIO in rats by inhibiting osteoclastogenesis and PTH secretion and stimulating osteoblast activity.
本研究在糖皮质激素诱导的骨质疏松症（GIO）大鼠模型中，检测了间歇性禁食（IF）对骨矿物质含量（BMC）、骨矿物质密度（BMD）以及骨重塑标志物的影响。
将40只雄性大鼠分为4组（每组n = 10）：正常大鼠对照组；对照+IF组（正常大鼠每日进行16 - 18小时间歇性禁食，持续90天）；地塞米松（DEX）组（腹腔注射DEX [0.5 mg]，持续90天）；以及DEX + IF组（DEX和IF处理90天）。实验结束时，测量右侧胫骨的BMD和BMC。检测以下血清水平：葡萄糖；胰岛素；甘油三酯（TGs）；总胆固醇；甲状旁腺激素（PTH）；骨保护素（OPG）；核因子κB受体激活剂（RANK）；骨吸收细胞因子，包括骨脱氧吡啶啉（DPD）、I型胶原N端前肽（NTX - 1）和抗酒石酸酸性磷酸酶5b（TRAP - 5b）；以及骨形成细胞因子，包括碱性磷酸酶（ALP）和骨钙素（OC）。
给予DEX 90天导致血清葡萄糖、胰岛素、TGs、胆固醇、PTH、OPG、DPD、NTX - 1和TRAP - 5b水平显著升高，BMD、BMC以及血清RANK、OC和ALP水平显著降低（均P < 0.05）。90天的IF显著改善了所有这些参数（均P < 0.05）。
IF通过抑制破骨细胞生成和PTH分泌以及刺激成骨细胞活性来纠正大鼠的GIO。
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