Gartner S, Markovits P, Markovitz D M, Betts R F, Popovic M
JAMA. 1986 Nov 7;256(17):2365-71.
Primary cultures from a brain biopsy specimen of a human T-cell lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) seropositive patient with progressive dementia contained small numbers of monocytoid cells and showed reverse transcriptase activity that persisted for as long as 100 days. Electron microscopy of these cells revealed the presence of HTLV-III/LAV virions. Subcultured cells removed from primary cultures by trypsinization were nonspecific esterase negative and did not express virus or show evidence of HTLV-III/LAV proviral sequences, while those remaining in the original flasks were nonspecific esterase positive and continued to produce virus. Virus from primary cultures was transmitted to peripheral blood-derived monocyte-macrophages and T cells. Virus production in T-cell cultures was transient while the monocyte-macrophages, like the primary cultures, produced virus for at least 120 days. Infection of several brain-derived cells with this and another HTLV-III/LAV isolate failed to demonstrate virus replication. These results indicate that the HTLV-III/LAV-infected cells recovered from the brain of this patient are cells of the mononuclear phagocyte series.
从一名患有进行性痴呆的人类嗜T细胞病毒III型/淋巴结病相关病毒（HTLV-III/LAV）血清阳性患者的脑活检标本中获得的原代培养物含有少量单核细胞样细胞，并显示出持续长达100天的逆转录酶活性。对这些细胞进行电子显微镜检查发现存在HTLV-III/LAV病毒颗粒。通过胰蛋白酶消化从原代培养物中分离出的传代培养细胞非特异性酯酶呈阴性，不表达病毒，也未显示HTLV-III/LAV前病毒序列的证据，而留在原始培养瓶中的细胞非特异性酯酶呈阳性，并继续产生病毒。来自原代培养物的病毒可传播至外周血来源的单核细胞-巨噬细胞和T细胞。T细胞培养物中的病毒产生是短暂的，而单核细胞-巨噬细胞与原代培养物一样，至少120天持续产生病毒。用该病毒和另一种HTLV-III/LAV分离株感染几种脑源性细胞未能证明病毒复制。这些结果表明，从该患者大脑中分离出的HTLV-III/LAV感染细胞是单核吞噬细胞系列的细胞。
