Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Department of Biomedical Science, College of Natural Sciences, Chosun University, Chosun, 61452, South Korea.
J Biomed Nanotechnol. 2021 Nov 1;17(11):2153-2164. doi: 10.1166/jbn.2021.3181.
In this approach, Hepatocellular carcinoma (HCC) is originated from hepatocytes cell, which can spread several parts in the body. It increases the death rate of cancer patients and more common in men rather than female. Patients having large tumor are growing through expensive treatment such as chemotherapy, radiotherapy and surgery. Nano medicine such as nano-dimensional particles as well as quantum dots might be an alternative treatment with greater efficiency in cancer biology field. Modification of surface and chemical properties of cadmium groups quantum dots can easily penetrate into the cancer cell without harming normal tissues. Here, Cadmium-Selenium Quantum Dot nanomaterials (CdSe QDs) have been prepared in solution phase with 0.1 M concentration, which was potentially applied for the destroying of HepG2 cancer cell with 24 hour and 36 hour of incubation. Due to their size, surface properties, lower cost, QDs can easily attached to the cell and able to damage the cells more rapidly process. For cell death, gene expression and morphological changing analysis were completed MTT, Flow Cytometry, qRT-PCR assay. Finally, the cell deaths were observed by cell shrinkage, rupture of membrane and expression of apoptotic gene (Bcl2, Beta catenin, Bax) were positive comparing untreated HepG2 cell line.
在这种方法中，肝细胞癌（HCC）起源于肝细胞，可以在体内的多个部位扩散。它增加了癌症患者的死亡率，而且在男性中比女性更为常见。患有大肿瘤的患者通过昂贵的治疗方法（如化疗、放疗和手术）进行治疗。纳米医学，如纳米级颗粒和量子点，可能是一种更有效的癌症生物学领域的替代治疗方法。通过对镉组量子点的表面和化学性质进行修饰，可以很容易地穿透癌细胞而不伤害正常组织。在这里，我们在溶液相中制备了硒化镉量子点（CdSe QDs）纳米材料，浓度为 0.1 M，该纳米材料有望在 24 小时和 36 小时孵育后用于破坏 HepG2 癌细胞。由于其尺寸、表面性质和较低的成本，QDs 可以很容易地附着在细胞上，并能够更快地破坏细胞。为了研究细胞死亡，我们完成了 MTT、流式细胞术和 qRT-PCR 检测，分析基因表达和形态变化。最后，通过细胞收缩、细胞膜破裂和凋亡基因（Bcl2、Beta catenin、Bax）的表达观察到细胞死亡，与未处理的 HepG2 细胞系相比呈阳性。
