Yan Sen, Tian Sinuo, Meng Zhiyuan, Sun Wei, Xu Ning, Jia Ming, Huang Shiran, Wang Yu, Zhou Zhiqiang, Diao Jinling, Zhu Wentao
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China.
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China; School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Environ Pollut. 2022 Feb 1;294:118676. doi: 10.1016/j.envpol.2021.118676. Epub 2021 Dec 11.
Although many toxicological studies on pesticides and nanoparticles have been conducted, it is not clear whether nanoparticles will increase the toxicity of pesticides. In this study, we chose imidacloprid (IMI) as a representative pesticide, and explored the influence of ZnO NPs on the toxic effect of IMI. In addition, we studied the bioaccumulation of IMI in mice. Using biochemical index analysis, liver histopathological analysis, non-targeted metabolomics, and LC/MS analysis, we found that ZnO NPs increased the toxicity of IMI, which may be related to the increase in IMI bioaccumulation in mice. In addition, we used intestinal histopathological analysis, RT-qPCR, and 16sRNA sequencing to find that the disturbance of the gut microbiota and the impaired intestinal barrier caused by ZnO NPs may be the reason for the increase in IMI bioaccumulation. In summary, our results indicate that ZnO NPs disrupted the intestinal barrier and enhanced the bioaccumulation of IMI, and therefore increased the toxicity of IMI in mice. Our research has deepened the toxicological insights between nanomaterials and pesticides.
尽管已经开展了许多关于农药和纳米颗粒的毒理学研究，但纳米颗粒是否会增加农药的毒性尚不清楚。在本研究中，我们选择吡虫啉（IMI）作为代表性农药，并探究了氧化锌纳米颗粒（ZnO NPs）对IMI毒性效应的影响。此外，我们研究了IMI在小鼠体内的生物蓄积情况。通过生化指标分析、肝脏组织病理学分析、非靶向代谢组学和液相色谱/质谱分析，我们发现ZnO NPs增加了IMI的毒性，这可能与小鼠体内IMI生物蓄积的增加有关。此外，我们通过肠道组织病理学分析、逆转录定量聚合酶链反应（RT-qPCR）和16sRNA测序发现，ZnO NPs引起的肠道微生物群紊乱和肠道屏障受损可能是IMI生物蓄积增加的原因。总之，我们的结果表明，ZnO NPs破坏了肠道屏障，增强了IMI的生物蓄积，因此增加了IMI对小鼠的毒性。我们的研究加深了对纳米材料与农药之间毒理学的认识。
