脆弱拟杆菌活菌计数：微滴技术的一种改进
Counts on viable Bacteroides fragilis: a modification of the microdroplet technique.
作者信息
Kelly M J
出版信息
J Hyg (Lond). 1978 Jun;80(3):385-9. doi: 10.1017/s0022172400024839.
Abstract
A modification of Sharpe's microdroplet counting technique is presented which permits viable counting of Bacteroides fragilis. Lysed horse blood was added to 0.1 soy digest agar droplets and 2% was found to be the optimal concentration which combined adequate intradroplet colony size with acceptable visibility on the counting screen of the Colworth Droplette machine.
摘要
本文介绍了一种对夏普微滴计数技术的改进方法，该方法可对脆弱拟杆菌进行活菌计数。将溶血马血添加到0.1%大豆消化琼脂微滴中，发现2%的浓度为最佳浓度，此浓度能使微滴内菌落大小适宜，且在科尔沃思滴液器的计数屏幕上具有可接受的可见度。
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