College of Food Science and Engineering, Ocean University of China, Qingdao 266003, R. P. China.
Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, R. P. China.
ACS Biomater Sci Eng. 2022 Jan 10;8(1):293-302. doi: 10.1021/acsbiomaterials.1c01215. Epub 2021 Dec 15.
Stimuli-responsive hydrogels have drawn increasing research interest in regenerative medicine due to their tunable molecular structures and functional properties for both providing a suitable microenvironment for wound healing and to serve as a sustainable therapeutic. Hence, we developed a stimuli-responsive drug-loaded hydrogel wound dressing for sustained, controlled release of the drug and accelerating wound healing. Hydrogel dressings with stimuli-responsive properties were prepared using carboxymethyl agarose (CMA) with various degrees of substitution and calcium ion crosslinking, followed by the loading of recombinant human epidermal growth factor (Rh-EGF) on the CMA hydrogel. Experimental results indicated that these hydrogel composites showed pH and temperature stimuli-responsive behaviors and released the encapsulated drug sustainedly in various release media. Moreover, the hydrogel dressings exhibited a porous network structure, stable physical properties, and excellent biocompatibility. The investigation showed that the Rh-EGF-loaded CMA hydrogel dressing significantly enhanced wound healing and reduced inflammatory responses by upregulating the transforming growth factor-beta, vascular endothelial growth factor, and cluster of differentiation 31 (CD31). These results confirm that Rh-EGF-loaded CMA hydrogel dressings possess potential application in accelerating wound healing and tissue regeneration.
刺激响应水凝胶由于其可调的分子结构和功能特性，在再生医学中引起了越来越多的研究兴趣，既能为伤口愈合提供合适的微环境，又能作为一种可持续的治疗方法。因此，我们开发了一种刺激响应载药水凝胶伤口敷料，用于药物的持续、控制释放，加速伤口愈合。使用具有不同取代度的羧甲基琼脂糖（CMA）和钙离子交联制备具有刺激响应特性的水凝胶敷料，然后将重组人表皮生长因子（Rh-EGF）负载到 CMA 水凝胶上。实验结果表明，这些水凝胶复合材料表现出 pH 值和温度刺激响应行为，并在各种释放介质中持续释放包封的药物。此外，水凝胶敷料还表现出多孔网络结构、稳定的物理性能和优异的生物相容性。研究表明，负载 Rh-EGF 的 CMA 水凝胶敷料通过上调转化生长因子-β、血管内皮生长因子和分化簇 31（CD31），显著促进了伤口愈合，降低了炎症反应。这些结果证实，负载 Rh-EGF 的 CMA 水凝胶敷料在加速伤口愈合和组织再生方面具有潜在的应用前景。
