通过体内 CRISPR/Cas9 传递将第二个“绿色革命”突变引入小麦中。
Introduction of a second "Green Revolution" mutation into wheat via in planta CRISPR/Cas9 delivery.
机构信息
Genome-Edited Crop Development Group, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai 3-1-3, Tsukuba, Ibaraki 305-8604, Japan.
Biotechnology Research Laboratories, Agri-Bio Research Center, Kaneka Corporation, Iwata, Shizuoka 438-0802, Japan.
出版信息
Plant Physiol. 2022 Mar 28;188(4):1838-1842. doi: 10.1093/plphys/kiab570.
Abstract
Direct delivery of CRISPR/Cas9 ribonucleoproteins into the shoot apical meristem via particle bombardment enabled introduction of a semidwarf1-orthologous mutation into an elite wheat variety.
摘要
通过粒子轰击将 CRISPR/Cas9 核糖核蛋白直接递送至茎尖分生组织，从而将一个 semidwarf1 同源突变引入到一个优良小麦品种中。
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