棉花中基因家族的全基因组分析揭示了它们在纤维发育和应激反应中的潜在作用。

Genome-wide analysis of the gene family in cotton reveals their potential roles in fiber development and responses to stress.

作者信息

Feng Jiajia, Chen Yi, Xiao Xianghui, Qu Yunfang, Li Pengtao, Lu Quanwei, Huang Jinling

机构信息

College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China.

School of Biotechnology and Food Engineering, Anyang Institute of Technology, Anyang, Henan, China.

出版信息

PeerJ. 2021 Nov 30;9:e12557. doi: 10.7717/peerj.12557. eCollection 2021.

Callose deposition occurs during plant growth and development, as well as when plants are under biotic and abiotic stress. is a key enzyme for the synthesis of callose. In this study, 27, 28, 16, and 15 family members were identified in , , , and using the sequence of . The were divided into five groups by phylogenetic, gene structure, and conservative motif analysis. The conserved motifs and gene structures of in each group were highly similar. Based on the analysis of cis-acting elements, it is inferred that were regulated by abiotic stress. WGD/Segmental duplication promoted the amplification of the gene in cotton, and purification selection had an important function in the family. The transcriptome data and qRT-PCR under cold, heat, salt, and PEG treatments showed that were involved in abiotic stress. The expression patterns of were different in various tissues. We predicted that , which was highly expressed in fibers, had an important effect on fiber elongation. Hence, these results help us understand the role of in fiber development and stress response.

胼胝质沉积发生在植物生长发育过程中，以及植物受到生物和非生物胁迫时。（此处原文缺失关键酶相关具体信息）是合成胼胝质的关键酶。在本研究中，利用（此处原文缺失具体序列信息）的序列，在（此处原文缺失具体物种信息）、（此处原文缺失具体物种信息）、（此处原文缺失具体物种信息）和（此处原文缺失具体物种信息）中分别鉴定出27个、28个、16个和15个（此处原文缺失关键酶相关具体信息）家族成员。通过系统发育、基因结构和保守基序分析，将这些（此处原文缺失关键酶相关具体信息）分为五组。每组中（此处原文缺失关键酶相关具体信息）的保守基序和基因结构高度相似。基于顺式作用元件分析，推测（此处原文缺失关键酶相关具体信息）受非生物胁迫调控。全基因组复制/片段重复促进了棉花中（此处原文缺失关键酶相关具体信息）基因的扩增，纯化选择在（此处原文缺失关键酶相关具体信息）家族中具有重要作用。冷、热、盐和聚乙二醇处理下的转录组数据和qRT-PCR表明，（此处原文缺失关键酶相关具体信息）参与非生物胁迫。（此处原文缺失关键酶相关具体信息）在不同组织中的表达模式不同。我们预测在纤维中高表达的（此处原文缺失关键酶相关具体信息）对纤维伸长有重要影响。因此，这些结果有助于我们了解（此处原文缺失关键酶相关具体信息）在纤维发育和胁迫响应中的作用。