Lebreton Francois, Snesrud Erik, Hall Lindsey, Mills Emma, Galac Madeline, Stam Jason, Ong Ana, Maybank Rosslyn, Kwak Yoon I, Johnson Sheila, Julius Michael, Ly Melissa, Swierczewski Brett, Waterman Paige E, Hinkle Mary, Jones Anthony, Lesho Emil, Bennett Jason W, McGann Patrick
Multidrug-Resistant Organism Repository and Surveillance Network (MRSN), Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Bacterial Disease Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
JAC Antimicrob Resist. 2021 Dec 10;3(4):dlab179. doi: 10.1093/jacamr/dlab179. eCollection 2021 Dec.
is a leading cause of community- and hospital-acquired infections. Successful treatment is hampered by its remarkable ability to rapidly develop resistance to antimicrobial agents, primarily through mutation. In response, WHO listed carbapenem-resistant as a Priority 1 (Critical) pathogen for research and development of new treatments. A key resource in developing effective countermeasures is access to diverse and clinically relevant strains for testing. Herein we describe a panel of 100 diverse strains to support this endeavour.
WGS was performed on 3785 isolates in our repository. Isolates were cultured from clinical samples collected from healthcare facilities around the world between 2003 and 2017. Core-genome MLST and high-resolution SNP-based phylogenetic analyses were used to select a panel of 100 strains that captured the genetic diversity of this collection. Antibiotic susceptibility testing was also performed using 14 clinically relevant antibiotics.
This 100-strain diversity panel contained representative strains from 91 different STs, including genetically distinct strains from major epidemic clones ST-111, ST-235, ST-244 and ST-253. Seventy-one distinct antibiotic susceptibility profiles were identified ranging from pan-susceptible to pan-resistant. Known resistance alleles as well as the most prevalent mutations underlying the antibiotic susceptibilities were characterized for all isolates.
This panel provides a diverse and comprehensive set of strains for use in developing solutions to antibiotic resistance. The isolates and available metadata, including genome sequences, are available to industry, academia, federal and other laboratories at no additional cost.
是社区获得性感染和医院获得性感染的主要原因。其通过突变迅速对抗菌药物产生耐药性的显著能力阻碍了成功治疗。作为应对措施，世界卫生组织将耐碳青霉烯类列为新治疗方法研发的优先1类（关键）病原体。开发有效对策的一个关键资源是获得用于测试的多样且临床相关的菌株。在此，我们描述了一组100种多样的菌株以支持这一努力。
对我们储存库中的3785株分离株进行了全基因组测序。分离株是从2003年至2017年期间从世界各地医疗机构收集的临床样本中培养出来的。使用核心基因组多位点序列分型和基于单核苷酸多态性的高分辨率系统发育分析来选择一组100株菌株，这些菌株捕获了该集合的遗传多样性。还使用14种临床相关抗生素进行了药敏试验。
这个100株菌株的多样性面板包含来自91个不同序列型的代表性菌株，包括来自主要流行克隆ST-111、ST-235、ST-244和ST-253的基因不同的菌株。鉴定出了从全敏感到全耐药的71种不同的药敏谱。对所有分离株的已知耐药等位基因以及药敏性背后最普遍的突变进行了表征。
该面板提供了一组多样且全面的菌株，用于开发抗生素耐药性的解决方案。这些分离株和可用的元数据，包括基因组序列，可供行业、学术界、联邦和其他实验室免费使用。
