Moran Mary C, Pandya Radha P, Leffler Kimberly A, Yoshida Takeshi, Beck Lisa A, Brewer Matthew G
Department of Microbiology & Immunology, University of Rochester Medical Center, Rochester, New York, USA.
Department of Dermatology, University of Rochester Medical Center, Rochester, New York, USA.
JID Innov. 2021 Apr 28;1(2):100018. doi: 10.1016/j.xjidi.2021.100018. eCollection 2021 Jun.
Epidermal cell models are critical for studying skin biology. The gold standard used by the scientific community has historically been primary cell cultures from discarded tissue, typically from neonates (foreskin). Although directly applicable to humans, this system suffers from multiple issues, including substantial donor-to-donor variability and a finite number of divisions in culture. As such, we have identified a faithful alternative called N/TERT2G cells. These cells show many of the characteristics of primary cells, including barrier formation, differentiation kinetics and/or protein expression, and pathogenesis. From our observations, N/TERT2G cells can serve as a reproducible and genetically manipulatable platform in studying skin biology.
表皮细胞模型对于研究皮肤生物学至关重要。科学界一直以来使用的金标准是来自废弃组织（通常是新生儿包皮）的原代细胞培养物。尽管该系统可直接应用于人类，但它存在多个问题，包括供体间的显著差异以及培养中有限的分裂次数。因此，我们已经确定了一种可靠的替代物，称为N/TERT2G细胞。这些细胞具有许多原代细胞的特征，包括屏障形成、分化动力学和/或蛋白质表达以及发病机制。根据我们的观察，N/TERT2G细胞可作为研究皮肤生物学的可重复且可进行基因操作的平台。
