长期刺激后附近非兴奋穴位处的激肽原-一氧化氮信号传导
Kininogen-Nitric Oxide Signaling at Nearby Nonexcited Acupoints after Long-Term Stimulation.
作者信息
Wang Ting, Zhu Geng, Qin Liyue, Wang Qian, She Chen, Xu Dongsheng, Hu Weiwei, Luo Kenghuo, Lei Ying, Gong Yanling, Ghosh Arijit, Ma Dongni, Ding Chun-Lei, Wang Bu-Yi, Guo Yang, Ma Shou-Shan, Hattori Michihiro, Takagi Yutaka, Ara Katsutoshi, Higuchi Kazuhiko, Li Xingwang, He Lin, Bai Wanzhu, Ishida Koichi, Li Sheng-Tian
机构信息
Bio-X Institutes, Key Laboratory for the Genetics of Development and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
Brain Science and Technology Research Center, Shanghai Jiao Tong University, Shanghai, China.
出版信息
JID Innov. 2021 Jun 24;1(3):100038. doi: 10.1016/j.xjidi.2021.100038. eCollection 2021 Sep.
Acupuncture treatment is based on acupoint stimulation; however, the biological basis is not understood. We stimulated one acupoint with catgut embedding for 8 weeks and then used isobaric tags for relative and absolute quantitation to screen proteins with altered expression in adjacent acupoints of Sprague Dawley rats. We found that kininogen expression was significantly upregulated in the stimulated and the nonstimulated adjacent acupoints along the same meridian. The enhanced kininogen expression was meridian dependent and was most apparent among small vessels in the subcutaneous layer. Enhanced signals of nitric oxide synthases, cGMP-dependent protein kinase, and myosin light chain were also observed at the nonstimulated adjacent acupoints along the same meridian. These findings uncover biological changes at acupoints and suggest the critical role of the kininogen-nitric oxide signaling pathway in acupoint activation.
针刺治疗基于穴位刺激；然而，其生物学基础尚不清楚。我们用埋线法刺激一只大鼠的一个穴位，持续8周，然后使用同量异位标签相对和绝对定量法来筛选在斯普拉格-道利大鼠相邻穴位中表达发生改变的蛋白质。我们发现，在受刺激穴位以及沿同一经络的未受刺激相邻穴位中，激肽原表达显著上调。激肽原表达的增强是经络依赖性的，在皮下层的小血管中最为明显。在沿同一经络的未受刺激相邻穴位中也观察到一氧化氮合酶、环磷酸鸟苷依赖性蛋白激酶和肌球蛋白轻链的信号增强。这些发现揭示了穴位处的生物学变化，并表明激肽原-一氧化氮信号通路在穴位激活中起关键作用。
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