Ma Sheng-Xing, Lee Paul C, Anderson Thomas L, Li Xi-Yan, Jiang Isabelle Z
Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center and Department of Obstetrics and Gynecology, David Geffen School of Medicine, University of California at Los Angeles, Torrance, CA 90502, USA.
Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center and Department of Neurology, David Geffen School of Medicine, University of California at Los Angeles, Torrance, CA 90502, USA.
Evid Based Complement Alternat Med. 2017;2017:4694238. doi: 10.1155/2017/4694238. Epub 2017 Jun 22.
This study was to examine the influences of manual acupuncture (MA) and electrical heat corresponding to reinforcing methods on nitric oxide (NO) release over the skin regions in humans. A device with collecting solution was taped to the skin surface along pericardium (PC) or lung (LU) meridian. Acupuncture needles were gently inserted into PC 4 with reinforcing stimulation (low force/rate) for 20 minutes in the MA group. LU11 on the finger was heated (43-44°C) by electrical heat for 20 minutes. Biocapture was consecutively conducted for two 20-minute intervals during and after each treatment. Total nitrite and nitrate (NO ) in the collecting samples were quantified using chemiluminescence in blinded fashion. Baseline NO levels are higher and tended to be higher over PC and LU acupoints during the 1st biocapture. NO levels over PC regions were consistently increased by MA during both intervals. NO concentrations over LU acupoints were increased and tended to be increased by electrical heat in the 1st and 2nd biocapture. The results suggest that reinforcing MA and electrical heat induce NO released from the local skin regions with higher levels at acupoints, which improve local circulation and contribute to the beneficial effects of the therapies.
本研究旨在探讨手针（MA）及与补法对应的电热刺激对人体皮肤区域一氧化氮（NO）释放的影响。将带有收集溶液的装置沿心包经（PC）或肺经（LU）贴于皮肤表面。在MA组中，将针刺轻轻刺入PC4并采用补法刺激（低力度/频率）20分钟。用电热对手指上的LU11加热（43 - 44°C）20分钟。在每次治疗期间及之后，连续进行两个20分钟的生物样品采集。采用化学发光法对收集样品中的总亚硝酸盐和硝酸盐（NO）进行盲法定量分析。在第一次生物样品采集期间，PC和LU穴位处的基线NO水平较高且有升高趋势。在两个时间段内，MA均使PC区域的NO水平持续升高。在第一次和第二次生物样品采集时，电热使LU穴位处的NO浓度升高且有升高趋势。结果表明，补法MA和电热刺激可诱导穴位处局部皮肤区域释放更高水平的NO，这改善了局部血液循环并促成了这些疗法的有益效果。
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