酿酒酵母尿素转运缺陷型菌株。
Urea transport-defective strains of Saccharomyces cerevisiae.
作者信息
Sumrada R, Gorski M, Cooper T
出版信息
J Bacteriol. 1976 Mar;125(3):1048-56. doi: 10.1128/jb.125.3.1048-1056.1976.
Abstract
Experiments characterizing the urea active transport system in Saccharomyces cerevisiae indicate that (i) formamide and acetamide are strong competitive inhibitors of urea accumulation, (ii) uptake is maximal at pH 3.3 and is 80% inhibited at pH 6.0, and (iii) adenosine 5'-triphosphate generated by glycolysis in conjunction with formation of an ion gradient is likely the driving force behind urea transport. Mutant strains were isolated that are unable to accumulate urea at external concentrations of 0.25 mM. These strains also exhibit a depressed growth rate on 10 mM urea, indicating existence of a relationship between the active transport and facilitated diffusion modes of urea uptake.
摘要
对酿酒酵母中尿素主动运输系统进行表征的实验表明
（i）甲酰胺和乙酰胺是尿素积累的强竞争性抑制剂；（ii）在pH 3.3时摄取量最大，在pH 6.0时摄取量受到80%的抑制；（iii）糖酵解产生的腺苷5'-三磷酸与离子梯度的形成共同作用，可能是尿素运输背后的驱动力。分离出了在外部尿素浓度为0.25 mM时无法积累尿素的突变菌株。这些菌株在10 mM尿素上也表现出生长速率降低的情况，这表明尿素摄取的主动运输和易化扩散模式之间存在关联。
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