Nikfarjam Mahdieh, Rashki Ghaleno Leila, Shahverdi Abdol Hossein, Mirshahvalad S Hahab, Ghoreishi Seyed Mehdi, Alizadeh Ali Reza
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Department of Cellular and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
Int J Fertil Steril. 2021 Oct;15(4):258-262. doi: 10.22074/IJFS.2021.141725.1052. Epub 2021 Oct 16.
Pomegranate is an ancient fruit containing Punicalagin, which has known as an effective antioxidant. Pomegranate peel was recognized as a phenol and tannin source, and pomegranate seed contains unique fatty acid (Punicic acid). Limited information exists about the influences of pomegranate peel and seed on antioxidant enzymes and proteins in the male reproduction system. This study was performed to determine the pomegranate peel and seed effects on the expression of antioxidant genes and DJ-1 protein in ram's testis.
In this experimental study, twenty-one mature Iranian rams were randomly divided into three groups (n=7 in each group), and fed experimental diets consisted of a control diet (C), a diet containing dry pomegranate seed pulp (S), and a diet containing pomegranate peel (P) for 80 days. All rams were offered isoenergetic and isonitrogenous rations. Testicular tissue samples were collected, and expression of , and genes was quantified by real-time polymerase chain reaction (RT-PCR). In addition, western blotting was used to evaluate DJ-1 expression at the protein level.
and mRNA levels in the peel group were significantly (P<0.05) higher than control. mRNA level was increased (P<0.05) in the seeds group than in the control group. and expression were statistically not affected significantly by the experimental diet. Data analysis showed a significant (P<0.05) increase (1.5-fold) in the expression level of DJ-1 in peel groups than in control.
The expression of antioxidant genes and DJ-1 protein in ram testes are more influenced by pomegranate peel than seed.
石榴是一种古老的水果，含有石榴皮素，这是一种已知的有效抗氧化剂。石榴皮被认为是酚类和单宁的来源，石榴籽含有独特的脂肪酸（石榴酸）。关于石榴皮和籽对雄性生殖系统中抗氧化酶和蛋白质的影响，现有信息有限。本研究旨在确定石榴皮和籽对公羊睾丸中抗氧化基因和DJ-1蛋白表达的影响。
在本实验研究中，将21只成熟的伊朗公羊随机分为三组（每组n = 7），并喂食实验日粮，包括对照日粮（C）、含干石榴籽果肉的日粮（S）和含石榴皮的日粮（P），持续80天。所有公羊均给予等能量和等氮日粮。采集睾丸组织样本，通过实时聚合酶链反应（RT-PCR）定量检测、和基因的表达。此外，采用蛋白质免疫印迹法在蛋白质水平评估DJ-1的表达。
果皮组和的mRNA水平显著高于对照组（P<0.05）。种子组的mRNA水平比对照组升高（P<0.05）。和的表达在统计学上不受实验日粮的显著影响。数据分析显示，果皮组中DJ-1的表达水平比对照组显著升高（P<0.05）（1.5倍）。
石榴皮对公羊睾丸中抗氧化基因和DJ-1蛋白表达的影响大于石榴籽。
