Rao Faiza, Tian Hui, Li Wenqing, Hung Helong, Sun Fei
Institute of Immunology and CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Biology, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, 230027; Department of Life Science, Hefei National Laboratory for Physical Sciences at Microscale, Hefei, 230027, China.
Asian J Androl. 2016 Jul-Aug;18(4):627-32. doi: 10.4103/1008-682X.168792.
Punicalagin is isolated from pomegranate and widely used for the treatment of different diseases in Chinese traditional medicine. This study aimed to evaluate the effect of Punicalagin (purity ≥98%) on oxidative stress induced testicular damage and its effect on fertility. We detected the antioxidant potential of punicalagin in lipopolysaccharide (LPS) induced oxidative stress damage in testes, also tried to uncover the boosting fertility effect of Punicalagin (PU) against oxidative stress-induced infertility. Results demonstrated that 9 mg kg-1 for 7 days treatment significantly decreases LPS induced oxidative damage in testes and nitric oxide production. The administration of oxidative stress resulted in a significant reduction in testes antioxidants GSH, T-SOD, and CAT raised LPO, but treatment with punicalagin for 7 days increased antioxidant defense GSH, T-SOD, and CAT by the end of the experiment and reduced LPO level as well. PU also significantly activates Nrf2, which is involved in regulation of antioxidant defense systems. Hence, the present research categorically elucidates the protective effect of punicalagin against LPS induced oxidative stress induced perturbation in the process of spermatogenesis and significantly increased sperm health and number. Moreover, fertility success significantly decreased in LPS-injected mice compared to controls. Mice injected with LPS had fertility indices of 12.5%, while others treated with a combination of PU + LPS exhibited 75% indices. By promoting fertility and eliminating oxidative stress and inflammation, PU may be a useful nutrient for the treatment of infertility.
石榴皮素是从石榴中分离出来的，在中国传统医学中被广泛用于治疗各种疾病。本研究旨在评估石榴皮素（纯度≥98%）对氧化应激诱导的睾丸损伤的影响及其对生育能力的作用。我们检测了石榴皮素在脂多糖（LPS）诱导的睾丸氧化应激损伤中的抗氧化潜力，还试图揭示石榴皮素（PU）对氧化应激诱导的不育症的促生育作用。结果表明，9毫克/千克体重连续7天给药可显著降低LPS诱导的睾丸氧化损伤和一氧化氮生成。氧化应激处理导致睾丸抗氧化剂谷胱甘肽（GSH）、总超氧化物歧化酶（T-SOD）和过氧化氢酶（CAT）显著减少，脂质过氧化物（LPO）升高，但石榴皮素连续7天治疗在实验结束时增加了抗氧化防御物质GSH、T-SOD和CAT，同时也降低了LPO水平。PU还显著激活了参与抗氧化防御系统调节的核因子E2相关因子2（Nrf2）。因此，本研究明确阐明了石榴皮素对LPS诱导的氧化应激在精子发生过程中引起的干扰具有保护作用，并显著提高了精子健康和数量。此外，与对照组相比，注射LPS的小鼠生育成功率显著降低。注射LPS的小鼠生育指数为12.5%，而其他用PU + LPS组合处理的小鼠生育指数为75%。通过促进生育和消除氧化应激及炎症，PU可能是一种治疗不育症的有用营养素。
