上调以抑制神经梅毒中IFN-γ表达并促进T细胞凋亡。
Upregulates to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis.
作者信息
Wu Kai-Xuan, Wang Xiao-Tong, Hu Xin-Lin, Jiang Xiao-Yong, Zhuang Jing-Cong, Xu Yan-Zhu, Lin Li-Rong, Tong Man-Li, Yang Tian-Ci, Liu Li-Li
机构信息
Center of Clinical Laboratory, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China.
Department of Dermatology, School of Medical, Zhongshan Hospital, Xiamen University, Xiamen, China.
出版信息
Front Microbiol. 2021 Nov 30;12:749171. doi: 10.3389/fmicb.2021.749171. eCollection 2021.
Long non-coding RNAs are involved in many infectious diseases. Our previous studies showed that expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether has biological functions remains unclear. The current study was undertaken to understand the mechanism of in T lymphocyte function in neurosyphilis patients. The pull-down assay showed that acted on the acetylase NAT10. The chromatin immunoprecipitation (ChIP)-PCR results showed that promoted the acetylation of histone H3K27 adjacent to the Kank1 promoter, thereby promoting Kank1 protein expression. Kank1 promotes 14-3-3 protein expression, inhibits NF-kB activation, inhibits IFN-γ secretion by T lymphocytes, and promotes T lymphocyte apoptosis. Taken together, our findings suggest a novel mechanism that upregulates to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis.
长链非编码RNA参与多种感染性疾病。我们之前的研究表明，与健康对照相比，神经梅毒患者的T淋巴细胞中[具体长链非编码RNA名称]表达增加。然而，[具体长链非编码RNA名称]是否具有生物学功能仍不清楚。当前研究旨在了解[具体长链非编码RNA名称]在神经梅毒患者T淋巴细胞功能中的作用机制。下拉实验表明，[具体长链非编码RNA名称]作用于乙酰化酶NAT10。染色质免疫沉淀（ChIP）-PCR结果表明，[具体长链非编码RNA名称]促进Kank1启动子附近组蛋白H3K27的乙酰化，从而促进Kank1蛋白表达。Kank1促进14-3-3蛋白表达，抑制NF-κB激活，抑制T淋巴细胞分泌IFN-γ，并促进T淋巴细胞凋亡。综上所述，我们的研究结果提示了一种新机制，即[具体长链非编码RNA名称]上调[具体相关分子名称]以抑制神经梅毒中IFN-γ表达并促进T细胞凋亡。
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