Cohen A, Boldur I, Tamarin-Landau R, Sompolinsky D
Isr J Med Sci. 1986 Sep;22(9):674-7.
The lungs of 139 deceased and 29 slaughtered calves were examined for Legionella by culture and by direct immunofluorescence (DIF) with fluorescein-conjugated antisera. About 17% of the cadaver lungs and 4% of lungs from slaughtered animals were positive by DIF, but only two L. pneumophila serogroup 1 strains were isolated from the lungs of two cadavers. In a prevalence study of antibodies to Legionella in domestic and wild animals of various species, titers of 1:64 were demonstrated by the indirect immunofluorescence technique in the sera of 10% of the dairy cattle, 5% of the beef cattle, 4% of the sheep, 22% of the antelopes, 35% of the horses, 36% of the water buffaloes and none of the laboratory rabbits. The isolation of Legionella from lung tissue is the strongest indication so far reported of the possible role of Legionella spp. in animal disease.
通过培养以及使用荧光素结合抗血清进行直接免疫荧光法（DIF），对139头死亡小牛和29头屠宰小牛的肺部进行了军团菌检测。通过DIF检测，约17%的尸体肺部和4%的屠宰动物肺部呈阳性，但仅从两具尸体的肺部分离出两株嗜肺军团菌血清1型菌株。在一项针对各种家养和野生动物军团菌抗体的流行率研究中，通过间接免疫荧光技术，在10%的奶牛、5%的肉牛、4%的绵羊、22%的羚羊、35%的马、36%的水牛血清中检测到效价为1:64的抗体，而实验用兔血清中未检测到。从肺组织中分离出军团菌是迄今为止所报道的军团菌属在动物疾病中可能起作用的最有力证据。
