Boldur I, Cohen A, Tamarin-Landau R, Sompolinsky D
Vet Microbiol. 1987 Apr;13(4):313-20. doi: 10.1016/0378-1135(87)90062-9.
The lungs of 139 calves presented for autopsy and 29 healthy slaughtered calves were examined for Legionella by culture and by direct immunofluorescence (DIF) with fluorescein-conjugated antisera. About 17% of the cadaver lungs and 4% of lungs from slaughtered animals were positive by DIF. Legionella organisms were only isolated from the lungs of two cadavers (L. pneumophila, serogroup 1). In a prevalence study of antibodies to Legionella in domestic and wild animals of various species, titers of greater than or equal to 64 were demonstrated by indirect immunofluorescence in sera of 10% of dairy cattle, 5% of beef cattle, 4% of sheep, 22% of antelopes, 35% of horses, 36% of buffaloes and 0% of laboratory rabbits. The isolation of Legionella from lung tissue is evidence for a possible etiologic role of Legionella spp. in natural pathology of animals.
对139头送检剖检的犊牛和29头健康屠宰犊牛的肺脏进行了军团菌检测，采用培养法以及使用荧光素标记抗血清的直接免疫荧光法（DIF）。通过DIF检测，约17%的尸体肺脏和4%的屠宰动物肺脏呈阳性。仅从两具尸体的肺脏中分离出军团菌（嗜肺军团菌血清1型）。在一项针对各种家养和野生动物军团菌抗体的流行率研究中，通过间接免疫荧光法发现，10%的奶牛、5%的肉牛、4%的绵羊、22%的羚羊、35%的马、36%的水牛和0%的实验兔血清中抗体滴度大于或等于64。从肺组织中分离出军团菌证明了军团菌属在动物自然病理学中可能具有病因学作用。
