Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI, USA.
Department of Obstetrics and Gynecology, Department of Physiology, Cancer Center, Medical College of Wisconsin, Milwaukee, WI, USA.
Methods Mol Biol. 2022;2424:247-254. doi: 10.1007/978-1-0716-1956-8_16.
Mouse models-xenograft models, syngeneic models (directly implanted or chemically or virally induced), and genetically engineered mice (including transgenic and knockout methods) are invaluable for preclinical studies of ovarian cancer as they recapitulate the structures and microenvironments of tumors, which in vitro studies are unable to accomplish.This chapter describes the methodology and approaches for generating various murine models currently employed in ovarian cancer research. It covers the implantation of cells from ovarian cancer cell lines into mice by intraperitoneal injection.
鼠模型-异种移植模型、同基因模型（直接植入或化学或病毒诱导）和基因工程小鼠（包括转基因和敲除方法）对于卵巢癌的临床前研究非常有价值，因为它们再现了肿瘤的结构和微环境，而体外研究无法实现这一点。本章描述了目前用于卵巢癌研究的各种鼠模型的生成方法和途径。它涵盖了通过腹腔注射将卵巢癌细胞系的细胞植入小鼠体内的过程。
