Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan.
Eur Rev Med Pharmacol Sci. 2021 Dec;25(23):7306-7316. doi: 10.26355/eurrev_202112_27424.
Arum hygrophilum Bioss is a plant native to Asia, Europe, and Northern Africa. It is consumed as beverages, spices, or cooked leaves to cure gastrointestinal infections and cancer. This study aims to determine the antibacterial and anticancer effectivenesss of A. hygrophilum Bioss.
Using the well-diffusion method, the antimicrobial activity of the plant's aqueous extract and five other organic extracts were evaluated against bacteria often associated with food poisoning. The assessment of the antiproliferative activity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was done on five cancerous cell lines and on fibroblasts as a reference cell line.
The growth of L. monocytogenes was significantly inhibited by the aqueous and ethanolic extracts. Both extracts had a minimum inhibitory concentration (MIC) of 62.5 mg/mL. The inhibition caused by the methanolic extract had a MIC of 500 mg/mL. The growth of S. aureus and MRSA were inhibited by the aqueous extract with a MIC of 500 mg/mL, while the inhibition caused by the ethanolic extract had a MIC of 250 mg/mL on MRSA and 500 mg/mL on S.aureus. Both strains of S.aureus were also inhibited by the 3-pentanon extract, while the butanol extract only exhibited a moderate growth inhibition against MRSA. The MTT assay showed that the aqueous extract had not affected the proliferation of cancer cell lines. The cytotoxicity of the ethanolic and methanolic extracts had no concentration-inhibition relationship and the IC50 values were above 800 μg/mL for all extracts.
L. monocytogenes, S. aureus, and methicillin-resistant Staphylococcus aureus (MRSA) were inhibited by different Arum extracts. The antibacterial activity of Arum hygrophilum Bioss against foodborne pathogens makes it safe to use as a natural food preservative, and as a source for sanitizers and antimicrobials. Further investigation is recommended to determine the cytotoxicity of the plant against additional cancer cell lines.
水芋是一种原产于亚洲、欧洲和北非的植物。人们将其作为饮料、香料或煮过的叶子食用，以治疗胃肠道感染和癌症。本研究旨在确定水芋的抗菌和抗癌作用。
采用琼脂扩散法，评估了植物水提物和其他五种有机提取物对常与食物中毒有关的细菌的抗菌活性。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估了 5 种癌细胞系和成纤维细胞（参考细胞系）的增殖抑制活性。
水提物和醇提物显著抑制了单核细胞增生李斯特菌的生长。两种提取物的最低抑菌浓度（MIC）均为 62.5mg/mL。甲醇提取物的 MIC 为 500mg/mL。水提物对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的 MIC 为 500mg/mL，而醇提物对 MRSA 的 MIC 为 250mg/mL，对金黄色葡萄球菌的 MIC 为 500mg/mL。两种金黄色葡萄球菌菌株也被 3-戊酮提取物抑制，而丁醇提取物仅对 MRSA 表现出中度生长抑制。MTT 法表明，水提物未影响癌细胞系的增殖。醇提物和甲醇提取物的细胞毒性与浓度无关，所有提取物的 IC50 值均高于 800μg/mL。
不同的水芋提取物抑制了单核细胞增生李斯特菌、金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌。水芋对食源性病原体的抗菌活性使其可安全用作天然食品防腐剂，也可作为消毒剂和抗菌剂的来源。建议进一步研究以确定该植物对其他癌细胞系的细胞毒性。
