Kariuki Francis, Getanda Pauline, Nyachieo Atunga, Juma Gerald, Kinyanjui Peter, Kamau Joseph
Department of Biochemistry, University of Nairobi, P.O. Box 30197-00100, Nairobi, Kenya.
Molecular Biology Unit, Institute of Primate Research, P.O. Box 24481-00502, Nairobi, Kenya.
Arch Microbiol. 2021 Dec 18;204(1):25. doi: 10.1007/s00203-021-02654-3.
Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. Lack of adequate diagnostic capabilities has contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. We evaluated the ability of staA, viaB and sopE genes to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis) using conventional polymerase chain reaction (PCR). The staA primers and viaB primers were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp, respectively. The sopE primers was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification with E. coli and S. boydii bacterial strains.
伤寒热由肠道沙门氏菌伤寒亚种血清型伤寒杆菌（伤寒杆菌）引起，在许多发展中国家仍然是一个严重的健康问题。缺乏足够的诊断能力在很大程度上导致了伤寒热在这些地区流行。需要可靠且廉价的诊断测试来改善对这种疾病负担的管理。我们使用常规聚合酶链反应（PCR）评估了staA、viaB和sopE基因检测和区分肯尼亚三种最常见沙门氏菌（伤寒杆菌、鼠伤寒沙门氏菌和肠炎沙门氏菌）的能力。发现staA引物和viaB引物仅对不同菌株的伤寒杆菌具有特异性，分别产生585 bp和540 bp的PCR产物。sopE引物被证明对所有沙门氏菌具有特异性，产生465 bp的PCR产物，对大肠杆菌和鲍氏志贺氏菌菌株无扩增。
Zotero 插件