外周血白细胞C3aR mRNA水平与血浆C3a、C3aR、IL-1β浓度及慢性阻塞性肺疾病急性加重之间的关联
Association between peripheral blood WBCs C3aR mRNA level and plasma C3a, C3aR, IL-1β concentrations and acute exacerbation of chronic obstructive pulmonary disease.
作者信息
Li Zhu, He Peiyong, Ding Hongwei, Gong Ling, Wu Jie, Zhong Chengyao, Liu Daishun
机构信息
Department of Respiratory Medicine, The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University), 563000 Guizhou, China.
The Third Hospital of Mianyang, Sichuan Mental Health Center, 621000 Sichuan, China.
出版信息
Immunobiology. 2022 Jan;227(1):152164. doi: 10.1016/j.imbio.2021.152164. Epub 2021 Dec 9.
BACKGROUND
The relationship between C3a-C3aR, IL-1β, and the acute exacerbation of chronic obstructive pulmonary disease is still unclear. This study aims to explore the expression levels of C3aR in peripheral blood WBCs and the concentrations of C3a, C3aR, and IL-1β in plasma in healthy controls and patients with chronic obstructive pulmonary disease (COPD).
METHODS
WBCs C3aR level in the peripheral blood, the concentrations of C3a, C3aR, and IL-1β in plasma were measured in 60 patients with acute exacerbation of COPD (AECOPD), 30 patients with stable COPD (SCOPD), and 30 healthy controls. The baseline characteristics and clinical data collected from enrolled patients, including age, gender, laboratory indicators, and lung function. We analyzed the correlation between C3a, C3aR, IL-1β, and lung function indicators (forced expiratory volume in the first second as a percentage of predicted value, FEV%pred) in the AECOPD group.
RESULTS
The white blood cell count (WBC), neutrophil/lymphocyte ratio (NLR), and C-reactive protein (CRP) of patients in COPD were higher than in healthy controls (P < 0.05). The peripheral blood WBCs C3aR mRNA and plasma C3a, C3aR, and IL-1β in AECOPD were higher than in SCOPD and healthy controls (P < 0.05). The peripheral blood WBCs C3aR mRNA and plasma C3aR, and IL-1β in AECOPD combined with respiratory failure were higher than in the non-respiratory failure group (P < 0.05). The peripheral blood WBCs C3aR mRNA and plasma C3a, C3aR, and IL-1β in AECOPD with high-risk were higher than in the low-risk group (P < 0.05). The peripheral blood WBCs C3aR mRNA and plasma C3a, C3aR, and IL-1β in AECOPD were negatively correlated with FEVpred%. The peripheral blood WBCs C3aR mRNA, the plasma C3a and C3aR in AECOPD were positively correlated with IL-1β.
CONCLUSION
The peripheral blood WBCs C3aR mRNA and plasma C3a, C3aR, and IL-1β in COPD patients were significantly related to the risk of disease deterioration. The C3a-C3aR axis may be involved in airway inflammation in patients with COPD.
背景
C3a - C3aR、白细胞介素 - 1β（IL - 1β）与慢性阻塞性肺疾病急性加重之间的关系仍不明确。本研究旨在探讨健康对照者和慢性阻塞性肺疾病（COPD）患者外周血白细胞中C3aR的表达水平以及血浆中C3a、C3aR和IL - 1β的浓度。
方法
测定60例慢性阻塞性肺疾病急性加重期（AECOPD）患者、30例稳定期COPD（SCOPD）患者和30例健康对照者外周血白细胞C3aR水平、血浆中C3a、C3aR和IL - 1β的浓度。收集纳入患者的基线特征和临床资料，包括年龄、性别、实验室指标和肺功能。分析AECOPD组中C3a、C3aR、IL - 1β与肺功能指标（第1秒用力呼气容积占预计值百分比，FEV%pred）之间的相关性。
结果
COPD患者的白细胞计数（WBC）、中性粒细胞/淋巴细胞比值（NLR）和C反应蛋白（CRP）高于健康对照者（P < 0.05）。AECOPD患者外周血白细胞C3aR mRNA以及血浆C3a、C3aR和IL - 1β高于SCOPD患者和健康对照者（P < 0.05）。合并呼吸衰竭的AECOPD患者外周血白细胞C3aR mRNA以及血浆C3aR和IL - 1β高于无呼吸衰竭组（P < 0.05）。高危AECOPD患者外周血白细胞C3aR mRNA以及血浆C3a、C3aR和IL - 1β高于低危组（P < 0.05）。AECOPD患者外周血白细胞C3aR mRNA以及血浆C3a、C3aR和IL - 1β与FEVpred%呈负相关。AECOPD患者外周血白细胞C3aR mRNA、血浆C3a和C3aR与IL - 1β呈正相关。
结论
COPD患者外周血白细胞C3aR mRNA以及血浆C3a、C3aR和IL - 1β与疾病恶化风险显著相关。C3a - C3aR轴可能参与COPD患者的气道炎症反应。
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