Nakamoto Y, Dohi K, Fujike H, Yuri T, Shinoda A, Takeuchi J
Kidney Int. 1978 Apr;13(4):297-305. doi: 10.1038/ki.1978.43.
Unilateral progressive Masugi nephritis was produced in rabbits and studied by microangiography as well as light and immunofluorescence microscopy. The following five groups were studied: Group 1. Heparin was started simultaneously with nephrotoxic serum (NTS) and was given for one week. The animals were then sacrificed along with the untreated controls. Group 2. This was the same protocol as in group 1 but with three weeks' heparin. Group 3. Heparin was started one day after NTS was given for three weeks. Group 4. Heparin was started one to two weeks after NTS and was given for three weeks. Group 5: late effect group. Heparin was started simultaneously with NTS, was given for four weeks, and the animals were then sacrificed 10 to 13 weeks later. Heparin dose was 5,000 U, s.c., per day in all treated groups. The number of glomeruli seen per unit of cortex by microangiography was significantly increased in the first through the third groups, as compared to the controls. Group 1 did not show this increase but there was some decrease of immunofluorescent fibrinogen. The late effect group (group 5) showed no modification by the treatment, suggesting that an initial improvement may have been negated by persistent immunologic insults after heparin withdrawal.
在兔子身上诱发了单侧进行性马杉肾炎，并通过微血管造影以及光学和免疫荧光显微镜进行研究。研究了以下五组：第1组。肝素与肾毒性血清（NTS）同时开始使用，持续给药一周。然后将这些动物与未治疗的对照组一起处死。第2组。这与第1组的方案相同，但肝素使用三周。第3组。在给予NTS一天后开始使用肝素，持续三周。第4组。在给予NTS一至两周后开始使用肝素，持续三周。第5组：延迟效应组。肝素与NTS同时开始使用，持续给药四周，然后在10至13周后将动物处死。所有治疗组的肝素剂量均为每天5000 U，皮下注射。与对照组相比，通过微血管造影在第一至第三组中每单位皮质中看到的肾小球数量显著增加。第1组未显示出这种增加，但免疫荧光纤维蛋白原有一定程度的减少。延迟效应组（第5组）未显示出治疗带来的改变，这表明在停用肝素后持续的免疫损伤可能抵消了最初的改善效果。
