Tissera Wernakulasooriya Manikkage Jayani Chethana Minoli, Rathnayake Suramya Indunil, Abeyrathne Edirisinghe Dewage Nalaka Sandun, Nam Ki-Chang
Department of Animal Science, Uva Wellassa University, Badulla, 90000 Sri Lanka.
Department of Science and Technology, Uva Wellassa University, Badulla, 90000 Sri Lanka.
Food Sci Biotechnol. 2021 Nov 26;30(13):1645-1655. doi: 10.1007/s10068-021-01002-x. eCollection 2021 Dec.
shell waste is highly accumulated in seafood processing factories and has low commercial applications. The objective of this study was to modify and develop a scale-up, simple, and high-yielding chemical method for extraction and purification of chitin and chitosan from shell waste. The developed method included a new "pretreatment" process using acetic acid followed by chemical treatments at each purification step. The final product was characterized by XRD and FTIR spectroscopy. Control chitin and chitosan were produced using a pre-described method for comparison. Yields of crude chitin, chitosan, and purified chitosan were 32.52 ± 0.68%, 26.28 ± 0.47%, and 21.78 ± 0.34% respectively whereas in the control chitin and chitosan the yields were 20.34 ± 0.72% and 13.79 ± 0.93% respectively ( < 0.05). Better physicochemical and functional properties were recorded in the developed method ( < 0.05). Hence the developed methodology can be scaled up and used in industrial applications.
The online version contains supplementary material available at 10.1007/s10068-021-01002-x.
贝壳废弃物在海产品加工厂中大量积累，且商业应用价值低。本研究的目的是改进并开发一种放大规模、简单且高产的化学方法，用于从贝壳废弃物中提取和纯化几丁质和壳聚糖。所开发的方法包括一个使用乙酸的新“预处理”过程，随后在每个纯化步骤进行化学处理。最终产物通过X射线衍射和傅里叶变换红外光谱进行表征。使用预先描述的方法制备对照几丁质和壳聚糖以作比较。粗几丁质、壳聚糖和纯化壳聚糖的产率分别为32.52±0.68%、26.28±0.47%和21.78±0.34%，而对照几丁质和壳聚糖的产率分别为20.34±0.72%和13.79±0.93%（P<0.05）。所开发的方法具有更好的物理化学和功能特性（P<0.05）。因此，所开发的方法可以放大规模并用于工业应用。
在线版本包含可在10.1007/s10068-021-01002-x获取的补充材料。
