Biswas S K
Lepr India. 1978 Jan;50(1):57-63.
A new approach in cultivation of Mycobacterium leprae in thyroxine treated Lowenstein-Jensen media is reported. The method has been proved to be successful as the organisms multiplied and remained viable in the thyroxine solution added at the bottom of the culture vial, for a period of 18 to 20 weeks of incubation at 37 degrees C. This has been possible due to metabolic stimulating action of thyroxine sodium solution as well as diffusion of nutrients into this solution from the medium. Intracutaneous inoculation of culture fluid on the surface of foot pad in cortisone treated mouse helped in rapid appearance of the specific lesion at the site within 20 days after injection. Both the intracutaneous method of inoculation and treatment of mouse with high doses of cortisone may play some role in shortening the period of development of such lesion.
据报道，在经甲状腺素处理的罗-琴培养基中培养麻风分枝杆菌有了一种新方法。该方法已被证明是成功的，因为在培养瓶底部添加的甲状腺素溶液中，这些微生物能够繁殖并保持活力，在37℃下孵育18至20周。这之所以成为可能，是由于甲状腺素钠溶液的代谢刺激作用以及营养物质从培养基扩散到该溶液中。在经可的松处理的小鼠脚垫表面皮内接种培养液，有助于在注射后20天内在该部位迅速出现特异性病变。皮内接种方法和用高剂量可的松处理小鼠可能在缩短此类病变的发展期方面都发挥了一定作用。
