Institute for Stroke and Dementia Research (ISD), University Hospital, LMU Munich, Munich, Germany.
Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.
STAR Protoc. 2021 Dec 1;2(4):100983. doi: 10.1016/j.xpro.2021.100983. eCollection 2021 Dec 17.
A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model. The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages. For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).
组织损伤（如中风和烧伤）的一个关键观察结果是全身免疫抑制状态，其特征是 T 细胞减少和感染增加。在这里，我们提出了一种固有（巨噬细胞）和适应性（T 细胞）免疫细胞之间细胞-细胞相互作用的模型。该方案促进了骨髓来源的巨噬细胞（BMDM）和脾 T 细胞在共培养模型中的共培养。该程序模拟了由巨噬细胞中炎症小体激活驱动的损伤诱导的 T 细胞死亡。有关此方案使用和执行的完整详细信息，请参阅 Roth 等人。（2021 年）。
