Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong, 518000, China.
College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, 518000, China.
Small Methods. 2021 Feb;5(2):e2000920. doi: 10.1002/smtd.202000920. Epub 2020 Dec 13.
The size and structural control of particulate carriers for imaging agents and therapeutics are constant themes in designing smart delivery systems. This is motivated by the causal relationship between geometric parameters and functionalities of delivery vehicles. Here, both in vitro and in vivo, the controlling factors for cytotoxicity, photothermal, and anti-tumor effects of biodegradable magnesium@poly(lactic-co-glycolic acid (Mg@PLGA) particulate carriers with different sizes and shell thicknesses are investigated. Mg@PLGA microspheres fabricated by microfluidic emulsification are shown to have higher Mg encapsulation efficiency, 87%, than nanospheres by ultrasonic homogenization, 50%. The photothermal and anti-tumor effects of Mg@PLGA spheres are found to be dictated by their Mg content, irrelevant to size and structural features, as demonstrated in both in vitro cell assays and in vivo mice models. These results also provide important implications for designing and fabricating stimuli-responsive drug delivery vehicles.
颗粒载体的大小和结构控制对于成像剂和治疗剂来说是设计智能输送系统的不变主题。这是由输送载体的几何参数和功能之间的因果关系所驱动的。在这里，无论是在体外还是体内，研究了不同尺寸和壳层厚度的生物可降解镁@聚（乳酸-共-羟基乙酸）（Mg@PLGA）颗粒载体的细胞毒性、光热和抗肿瘤作用的控制因素。通过微流乳化制备的 Mg@PLGA 微球比超声匀浆法制备的纳米球具有更高的 Mg 封装效率，达到 87%，而超声匀浆法的封装效率为 50%。Mg@PLGA 球体的光热和抗肿瘤作用被发现取决于它们的 Mg 含量，与尺寸和结构特征无关，这在体外细胞实验和体内小鼠模型中都得到了证明。这些结果也为设计和制造刺激响应型药物输送载体提供了重要的启示。
