State Key Laboratory of Pharmaceutical Biotechnology, Medical School & School of Life Sciences, Nanjing University, Nanjing 210093, China.
Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing 210023, China.
Nano Lett. 2022 Jan 12;22(1):229-237. doi: 10.1021/acs.nanolett.1c03693. Epub 2021 Dec 20.
Oxidative stress induced by hyperglycemia or chronic inflammation can limit diabetic wound healing, resulting in diabetic foot ulcers. Hydrogen has the potential to act as an antioxidant and scavenge reactive oxygen species, thereby attenuating inflammation in these chronic wounds. However, most of the reported H delivery systems for wound healing, including hydrogen gas, hydrogen-rich water, and hydrogen-rich saline, are very short-lived for the low solubility of hydrogen gas. Here, we introduce a hydrogen-producing hydrogel made of living and bacteria within a cell-impermeable casing that can continuously produce hydrogen for 60 h. This microbe-hydrogel system can selectively reduce highly toxic •OH and ONOO species and reduce inflammation. Additional experiments indicated that the microbe-hydrogel dressing could promote cell proliferation and diabetic wound healing by almost 50% at day 3. The symbiotic algae-bacteria hydrogel has excellent biocompatibility and reactive oxygen species scavenging features, indicating it has great promise for clinical use.
高血糖或慢性炎症引起的氧化应激会限制糖尿病伤口愈合，导致糖尿病足溃疡。氢气具有充当抗氧化剂和清除活性氧的潜力，从而减轻这些慢性伤口的炎症。然而，大多数报道的用于伤口愈合的氢气输送系统，包括氢气、富氢水和富氢盐水，由于氢气的低溶解度，其寿命都非常短。在这里，我们介绍了一种由活细菌和细胞不可渗透壳体内的细菌组成的产氢水凝胶，它可以持续产生氢气 60 小时。这种微生物-水凝胶系统可以选择性地还原高毒性的 •OH 和 ONOO 物质，并减少炎症。额外的实验表明，微生物水凝胶敷料可以促进细胞增殖，并使糖尿病伤口在第 3 天愈合速度加快近 50%。共生藻类-细菌水凝胶具有良好的生物相容性和活性氧清除特性，表明它具有很大的临床应用前景。
