Zhao Pengxiang, Li Yufei, Guo Boyuan, Liu Ziyi, Zhang Xujuan, Liu Mengyu, Ma Xuemei
College of Chemistry and Life Sciences, Beijing University of Technology, Beijing 100124, China.
Pharmaceutics. 2025 Feb 20;17(3):279. doi: 10.3390/pharmaceutics17030279.
: Wound healing is a complex and intricate biological process that involves multiple systems within the body and initiates a series of highly coordinated responses to repair damage and restore integrity and functionality. We previously identified that breathing hydrogen can significantly inhibit early inflammation, activate autologous stem cells, and promote the accumulation of extracellular matrix (ECM). However, the broader functions and downstream targets of hydrogen-induced ECM accumulation and tissue remodeling are unknown in the wound-healing process. : Consequently, this thesis developed a hydrogen sustained-release dressing based on a micro storage material and reveals the mechanism of hydrogen in treating wound healing. Upon encapsulating the hydrogen storage materials, magnesium (Mg), and ammonia borane (AB), we found that SiO@Mg exhibits superior sustained-release performance, while SiO@AB demonstrates a higher hydrogen storage capacity. We used a mouse full-thickness skin defect wound model to analyze and compare different hydrogen dressings. : It was identified that hydrogen dressings can significantly improve the healing rate of wounds by promoting epithelialization, angiogenesis, and collagen accumulation in wound tissue, and that the effect of slow-release dressings is better than of non-slow-release dressings. We also found that hydrogen dressing can promote transcriptome-level expression related to cell proliferation and differentiation and ECM accumulation, mainly through the pathway and pathway. : Overall, these results provide a novel insight into the field of hydrogen treatment and wound healing.
伤口愈合是一个复杂且精细的生物学过程，涉及体内多个系统，并引发一系列高度协调的反应来修复损伤、恢复完整性和功能。我们之前发现呼吸氢气可以显著抑制早期炎症、激活自体干细胞并促进细胞外基质（ECM）的积累。然而，在伤口愈合过程中，氢气诱导的ECM积累和组织重塑的更广泛功能及下游靶点尚不清楚。因此，本论文基于微存储材料开发了一种氢气缓释敷料，并揭示了氢气在治疗伤口愈合中的作用机制。在封装储氢材料镁（Mg）和氨硼烷（AB）后，我们发现SiO@Mg表现出优异的缓释性能，而SiO@AB具有更高的储氢容量。我们使用小鼠全层皮肤缺损伤口模型来分析和比较不同的氢气敷料。结果表明，氢气敷料可通过促进伤口组织的上皮形成、血管生成和胶原蛋白积累，显著提高伤口愈合率，且缓释敷料的效果优于非缓释敷料。我们还发现，氢气敷料主要通过 途径和 途径促进与细胞增殖、分化及ECM积累相关的转录组水平表达。总体而言，这些结果为氢气治疗和伤口愈合领域提供了新的见解。
