Collage of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, China.
Key Laboratory of Functional Nanomaterials and Technology in Universities of Shandong, Linyi University, Linyi, China.
J Nanobiotechnology. 2019 Mar 13;17(1):38. doi: 10.1186/s12951-019-0465-3.
Rapid and sensitive detection of HO especially endogenous HO is of great importance for series of industries including disease diagnosis and therapy. In this work, uniform FePt nanoparticles are successfully anchored onto Few-layer molybdenum disulfide nanosheets (F-MoS NSs). The powder X-ray diffraction, transmission electron microscopy, UV-Vis spectra and atomic force microscopy were employed to confirm the structure of the obtained nanocomposites (F-MoS-FePt NCs). The prepared nanocomposites show efficient peroxidase-like catalytic activities verified by catalyzing the peroxidation substrate 4,4'-diamino-3,3',5,5'-tetramethylbiphenyl (TMB) with the existence of HO.
The optimal conditions of the constructed colorimetric sensing platform is proved as 35 °C and pH 4.2. Under optimal catalytic conditions, the detection limit for HO detection reaches 2.24 μM and the linear ranger is 8 μM to 300 μM. Furthermore, the proposed colorimetric sensing platform was successfully utilized to detect the intracellular HO of cancer cells (MCF-7).
These findings indicated that the F-MoS-FePt-TMB-HO system provides a potential sensing platform for hydrogen peroxide monitoring in living cells.
快速、灵敏地检测 HO，特别是内源性 HO，对包括疾病诊断和治疗在内的一系列行业具有重要意义。在这项工作中，成功地将均匀的 FePt 纳米颗粒锚定在少层二硫化钼纳米片（F-MoS NSs）上。采用粉末 X 射线衍射、透射电子显微镜、紫外-可见光谱和原子力显微镜来确认所得纳米复合材料（F-MoS-FePt NCs）的结构。所制备的纳米复合材料通过催化过氧化物底物 4,4'-二氨基-3,3',5,5'-四甲基联苯（TMB）在 HO 的存在下表现出高效的过氧化物酶样催化活性。
所构建的比色传感平台的最佳条件被证明为 35°C 和 pH 4.2。在最佳催化条件下，对 HO 的检测限达到 2.24 μM，线性范围为 8 μM 至 300 μM。此外，所提出的比色传感平台成功地用于检测癌细胞（MCF-7）中的内源性 HO。
这些发现表明，F-MoS-FePt-TMB-HO 体系为活细胞中过氧化氢的监测提供了一种潜在的传感平台。
