Kawanishi H
Immunology. 1987 Jan;60(1):19-28.
In order to study the activity of immunocompetent lymphocytes in normal human adult mucosal lymphoid tissues in the gut, viable mononuclear cells (MNC) from surgically removed appendices were isolated with a rapid and improved technique, and their immune reactivity determined in vitro. Nearly two-thirds of the isolated cells were B cells, composed of sIgM (30%), sIgA (19%) and sIgG (12%), less than one-third of cells being T cells, composed of OKT4+ helper/inducer T cells (18%) and OKT8+ suppressor/cytotoxic T cells (9%). Isolated B cells were responsive to SAC (Cowan type I Staphylococcus aureus) with marked augmentation of IgG production, but not to lipopolysaccharides (LPS). In the presence or absence of pokeweed mitogen (PWM) and protein A, all isotype-specific Ig production was augmented along with increasing ratios of autologous T cells to B cells, whereas in the presence of anti-HLA-DR antibodies the production of IgA and IgM was reduced to a great extent. Finally, in vitro induction of concanavalin A (Con A)-induced suppressor T (Ts) cells was less than that of helper T (Th) cells. Thus, normal adult appendiceal (Ap) lymphocyte reactivity is predominated by helper T (Th) cells; IgA isotype-specific Ig production appears to be largely regulated by the autoreactive Th subset. This responsiveness to exogenous stimuli may play an important role in the gut mucosal immune responsiveness.
为了研究正常成年人体内肠道黏膜淋巴组织中免疫活性淋巴细胞的活性，采用一种快速改良技术从手术切除的阑尾中分离出有活力的单核细胞（MNC），并在体外测定其免疫反应性。分离出的细胞中近三分之二为B细胞，由sIgM（30%）、sIgA（19%）和sIgG（12%）组成，不到三分之一的细胞为T细胞，由OKT4 + 辅助/诱导性T细胞（18%）和OKT8 + 抑制/细胞毒性T细胞（9%）组成。分离出的B细胞对金黄色葡萄球菌 Cowan I型（SAC）有反应，IgG产生显著增加，但对脂多糖（LPS）无反应。在有或无商陆有丝分裂原（PWM）和蛋白A的情况下，随着自体T细胞与B细胞比例的增加，所有同种型特异性Ig的产生均增加，而在抗HLA - DR抗体存在的情况下，IgA和IgM的产生在很大程度上减少。最后，体外诱导的刀豆蛋白A（Con A）诱导的抑制性T细胞（Ts）少于辅助性T细胞（Th）。因此，正常成人阑尾（Ap）淋巴细胞反应性以辅助性T细胞（Th）为主；IgA同种型特异性Ig的产生似乎在很大程度上受自身反应性Th亚群调节。这种对外源刺激的反应性可能在肠道黏膜免疫反应中起重要作用。
