Dosch H M, Schuurman R K, Gelfand E W
J Immunol. 1980 Aug;125(2):827-32.
The capacity of the T cell mitogens phytohemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and Staphylococcus protein A (SpA) to induce B cell proliferation and differentiation was compared with the B cell mitogen, formalinized Staphylococcus aureus (STA). Lymphocyte subpopulations from normal donors and patients with various immunodeficiency diseases were studied. In the presence of the T cell mitogens, irradiated T cells were capable of providing a helper cell activity that enabled co-cultured B lymphocytes to proliferate in response to these mitogens and to differentiate into IgM-secreting (direct) hemolytic plaque-forming cells (PFC). In the PFC response, radioresistant T-helper and radiosensitive T-suppressor cell activities could be demonstrated. T-suppressor cell activity outweighed helper activity only in nonirradiated co-cultures stimulated with Con A. Patients with severe combined immunodeficiency lacked mitogen-induced helper T cells, whereas patients with various forms of humoral immune deficiency were normal in this respect. These findings and the tissue distribution of the helper activity is aquired early in post-thymic T cell differentiation. The data suggest that experiments with cell lineage-specific lymphocyte mitogens should be considered in the context of more complex cell-cell interactions.
将T细胞促有丝分裂原植物血凝素（PHA）、刀豆球蛋白A（Con A）、商陆有丝分裂原（PWM）和葡萄球菌蛋白A（SpA）诱导B细胞增殖和分化的能力与B细胞促有丝分裂原甲醛化金黄色葡萄球菌（STA）进行了比较。对来自正常供体和患有各种免疫缺陷疾病患者的淋巴细胞亚群进行了研究。在T细胞促有丝分裂原存在的情况下，经辐照的T细胞能够提供辅助细胞活性，使共培养的B淋巴细胞能够对这些促有丝分裂原作出反应而增殖，并分化为分泌IgM的（直接）溶血空斑形成细胞（PFC）。在PFC反应中，可以证明存在抗辐射的T辅助细胞和辐射敏感的T抑制细胞活性。仅在用Con A刺激的未辐照共培养物中，T抑制细胞活性超过辅助细胞活性。严重联合免疫缺陷患者缺乏促有丝分裂原诱导的辅助性T细胞，而各种形式的体液免疫缺陷患者在这方面是正常的。这些发现以及辅助活性的组织分布是在胸腺后T细胞分化早期获得的。数据表明，在更复杂的细胞间相互作用的背景下，应考虑使用细胞谱系特异性淋巴细胞促有丝分裂原进行实验。
