一种交叉反应性抗独特型抗体在体外对抗DNA抗体合成的抑制作用。
Suppression of anti-DNA antibody synthesis in vitro by a cross-reactive antiidiotypic antibody.
作者信息
Epstein A, Greenberg M, Diamond B, Grayzel A I
出版信息
J Clin Invest. 1987 Mar;79(3):997-1000. doi: 10.1172/JCI112912.
Abstract
An understanding of the regulatory mechanisms that govern the humoral immune response will be facilitated by the availability of techniques for the measurement of specific antibody production in vitro. We have developed an enzyme-linked immunosorbent assay system for the measurement of in vitro anti-DNA antibody production by peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus. Using this technique, the PBMC from 74% of serologically active SLE patients produced levels of anti-DNA antibodies that were greater than 2 SD above the mean of 18 +/- 9 IU/ml for normal subjects. Furthermore, the addition of 3I, a monoclonal anti-idiotypic antibody that recognizes a cross-reactive determinant on anti-DNA antibodies, was shown to specifically inhibit anti-DNA production in vitro.
摘要
体外测量特异性抗体产生的技术有助于深入了解调节体液免疫反应的调控机制。我们开发了一种酶联免疫吸附测定系统，用于测量系统性红斑狼疮患者外周血单个核细胞（PBMC）体外抗DNA抗体的产生。使用该技术，74%血清学活性SLE患者的PBMC产生的抗DNA抗体水平高于正常受试者18 +/- 9 IU/ml平均值的2个标准差。此外，添加3I，一种识别抗DNA抗体上交叉反应决定簇的单克隆抗独特型抗体，可在体外特异性抑制抗DNA的产生。
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