使用单克隆抗体鉴定系统性红斑狼疮患者针对天然DNA的人抗体上的共同独特型。
Use of monoclonal antibodies to identify shared idiotypes on human antibodies to native DNA from patients with systemic lupus erythematosus.
作者信息
Solomon G, Schiffenbauer J, Keiser H D, Diamond B
出版信息
Proc Natl Acad Sci U S A. 1983 Feb;80(3):850-4. doi: 10.1073/pnas.80.3.850.
Antinative DNA antibodies were purified from the serum of a patient with active systemic lupus erythematosus. Using hybridoma technology, we produced several mouse monoclonal antibodies directed against idiotypic determinants on these antinative DNA antibodies. One of these monoclonal anti-idiotypic antibodies, 3I, an IgG2a Kappa, was extensively characterized. 3I is believed to be directed against an idiotypic determinant because (i) its reactivity with antinative DNA antibodies is not inhibited by a large excess of pooled human serum, (ii) it reacts with F(ab')2 fragments of antinative DNA antibodies, and (iii) it reacts with antinative DNA antibodies of all IgG subclasses. 3I is not directed against the antigen binding site in that native DNA and 3I do not compete for binding to antinative DNA antibodies. Eight of nine sera containing antinative DNA activity as determined by the Millipore filter assay reacted with 3I in a solid-phase radioimmunoassay. These findings suggest that antinative DNA antibodies from nonrelated patients with systemic lupus erythematosus share a common idiotype.
从一名患有活动性系统性红斑狼疮患者的血清中纯化出抗天然DNA抗体。利用杂交瘤技术，我们制备了几种针对这些抗天然DNA抗体独特型决定簇的小鼠单克隆抗体。其中一种单克隆抗独特型抗体3I，属于IgG2a κ型，已被广泛鉴定。3I被认为是针对一种独特型决定簇，原因如下：（i）其与抗天然DNA抗体的反应性不会被大量过量的混合人血清所抑制；（ii）它能与抗天然DNA抗体的F(ab')2片段发生反应；（iii）它能与所有IgG亚类的抗天然DNA抗体发生反应。3I并不针对抗原结合位点，因为天然DNA和3I不会竞争与抗天然DNA抗体的结合。通过微孔滤膜分析确定具有抗天然DNA活性的9份血清中有8份在固相放射免疫分析中与3I发生反应。这些发现表明，来自非亲属系统性红斑狼疮患者的抗天然DNA抗体具有共同的独特型。
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